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Protein Page:
Hck (human)
rdtyret
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Hck a protein-tyrosine kinase of the Src family that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms that have different subcellular localization. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; EC 2.7.10.2; Oncoprotein; TK group; Src family
Chromosomal Location of Human Ortholog: 20q11-q12
Cellular Component: actin filament; caveola; cell projection; cytosol; extrinsic to internal side of plasma membrane; focal adhesion; Golgi apparatus; lysosome; nucleus; transport vesicle
Molecular Function: ATP binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein-tyrosine kinase activity; receptor binding
Biological Process: cell adhesion; cell differentiation; cytokine and chemokine mediated signaling pathway; inflammatory response; innate immune response; innate immune response-activating signal transduction; integrin-mediated signaling pathway; leukocyte degranulation; leukocyte migration during immune response; lipopolysaccharide-mediated signaling pathway; mesoderm development; negative regulation of apoptosis; peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; positive regulation of cell proliferation; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of cell shape; regulation of defense response to virus by virus; regulation of inflammatory response; regulation of phagocytosis; regulation of transcription factor activity; respiratory burst after phagocytosis; transmembrane receptor protein tyrosine kinase signaling pathway; viral reproduction
Reference #:  P08631 (UniProtKB)
Alt. Names/Synonyms: HCK; Hemopoietic cell kinase; JTK9; p59-HCK/p60-HCK; Tyrosine-protein kinase HCK
Gene Symbols: HCK
Molecular weight: 59,600 Da
Basal Isoelectric point: 6.27  Predict pI for various phosphorylation states
CST Pathways:  GPCR Signaling to MAPKs  |  Tyrosine Kinases & Substrates
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Hck

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
cell growth, altered: Y522‑p
activity, inhibited: Y522‑p

Modification Sites and Domains  
Click here to view other types of protein modifications

Modification Sites in Parent Protein, Orthologs, and Isoforms  
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 K28‑ub RMGCMKSkFLQVGGN
0 2 N35 kFLQVGGNtFSKTET
0 2 T36‑p FLQVGGNtFSKTETS
1 74 Y51‑p ASPHCPVyVPDPTST
0 1 Y87 DIIVVALYDyEAIHH
0 2 Y89‑p IVVALYDyEAIHHED
0 1 K124‑ub ARSLATRkEGyIPSN
0 1 Y127‑p LATRkEGyIPSNYVA
0 1 Y180‑p SETTKGSySLSVRDY
0 1 T202‑p VKHYKIRtLDNGGFy
0 424 Y209‑p tLDNGGFyISPRSTF
0 1 K228‑ac ELVDHYKkGNDGLCQ
0 1 S238‑p DGLCQKLsVPCMSSK
0 1 K283‑ub VWMATYNkHTkVAVK
0 1 K286‑ub ATYNkHTkVAVKTMK
0 1 T311‑p AEANVMKtLQHDKLV
0 7 Y330‑p VVTKEPIyIITEFMA
4 2119 Y411‑p RVIEDNEytAREGAK
0 13 T412‑p VIEDNEytAREGAKF
0 1 S442‑p TIKSDVWsFGILLME
0 1 S462‑p RIPYPGMsNPEVIRA
0 7 S520‑p DFYTATEsQyQQQP_
3 327 Y522‑p YTATEsQyQQQP___
  Hck iso2  
K7 _MGCMKSKFLQVGGN
N14 KFLQVGGNTFSKTET
T15 FLQVGGNTFSKTETS
Y30 ASPHCPVYVPDPTST
Y66 DIIVVALYDYEAIHH
Y68 IVVALYDYEAIHHED
K103 ARSLATRKEGYIPSN
Y106 LATRKEGYIPSNYVA
Y159 SETTKGSYSLSVRDY
T181 VKHYKIRTLDNGGFY
Y188 TLDNGGFYISPRSTF
K207 ELVDHYKKGNDGLCQ
S217 DGLCQKLSVPCMSSK
K262 VWMATYNKHTKVAVK
K265 ATYNKHTKVAVKTMK
T290 AEANVMKTLQHDKLV
Y309 VVTKEPIYIITEFMA
Y390 RVIEDNEYTAREGAK
T391 VIEDNEYTAREGAKF
S421 TIKSDVWSFGILLME
S441 RIPYPGMSNPEVIRA
S499 DFYTATESQYQQQP_
Y501 YTATESQYQQQP___
  Hck iso3  
K7 _MGCMKSKFLQVGGN
N14 KFLQVGGNTFSKTET
T15 FLQVGGNTFSKTETS
Y30 ASPHCPVYVPDPTST
Y65 DIIVVALYDYEAIHH
Y67 IVVALYDYEAIHHED
K102 ARSLATRKEGYIPSN
Y105 LATRKEGYIPSNYVA
Y158 SETTKGSYSLSVRDY
T180 VKHYKIRTLDNGGFY
Y187 TLDNGGFYISPRSTF
K206 ELVDHYKKGNDGLCQ
S216 DGLCQKLSVPCMSSK
K261 VWMATYNKHTKVAVK
K264 ATYNKHTKVAVKTMK
T289 AEANVMKTLQHDKLV
Y308 VVTKEPIYIITEFMA
Y389 RVIEDNEYTAREGAK
T390 VIEDNEYTAREGAKF
S420 TIKSDVWSFGILLME
S440 RIPYPGMSNPEVIRA
S498 DFYTATESQYQQQP_
Y500 YTATESQYQQQP___
  Hck iso4  
K28 RMGCMKSKFLQVGGN
N35 KFLQVGGNTFSKTET
T36 FLQVGGNTFSKTETS
Y51 ASPHCPVYVPDPTST
Y86 DIIVVALYDYEAIHH
Y88 IVVALYDYEAIHHED
K123 ARSLATRKEGYIPSN
Y126 LATRKEGYIPSNYVA
Y179 SETTKGSYSLSVRDY
T201 VKHYKIRTLDNGGFY
Y208 TLDNGGFYISPRSTF
K227 ELVDHYKKGNDGLCQ
S237 DGLCQKLSVPCMSSK
K282 VWMATYNKHTKVAVK
K285 ATYNKHTKVAVKTMK
T310 AEANVMKTLQHDKLV
Y329 VVTKEPIYIITEFMA
Y410 RVIEDNEYTAREGAK
T411 VIEDNEYTAREGAKF
S441 TIKSDVWSFGILLME
S461 RIPYPGMSNPEVIRA
S519 DFYTATESQYQQQP_
Y521 YTATESQYQQQP___
  mouse

 
R28 RMGCVKSRFLRDGsK
S34‑p SRFLRDGsKASKTEP
A36 FLRDGsKASKTEPSA
Y50‑p ANQKGPVyVPDPTSS
Y85‑p DTIVVALyDYEAIHR
Y87 IVVALyDYEAIHRED
K122 ARSLATKKEGYIPSN
Y125 LATKKEGYIPSNYVA
Y178 SETTKGSYSLSVRDF
T200 VKHYKIRTLDSGGFy
Y207‑p TLDSGGFyISPRSTF
K226 ELVLHYKKGKDGLCQ
S236 DGLCQKLSVPCVSPK
K281 VWMATYNKHTKVAVK
K284 ATYNKHTKVAVKTMK
S309 AEANLMKSLQHDKLV
F328 VVSQEPIFIVTEFMA
Y409‑p RIIEDNEytAREGAK
T410‑p IIEDNEytAREGAKF
S440 TIKSDVWSFGILLME
S460 RIPYPGMSNPEVIRA
S518 DFYTATESQyQQQP_
Y520‑p YTATESQyQQQP___
2101 : Phospho-Src Family (Tyr416) Antibody
6943 : Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb
  rat

 
R28 RMGCVKSRFLREGSK
S34 SRFLREGSKASKIEP
A36 FLREGSKASKIEPNA
Y50 ANQKGPVYVPDPTSP
Y85 DTIVVALYDYEAIHR
Y87 IVVALYDYEAIHRED
K122 ARSLATKKEGYIPSN
Y125 LATKKEGYIPSNYVA
Y178 SETTKGSYSLSVRDF
T200 VKHYKIRTLDSGGFy
Y207‑p TLDSGGFyISPRSTF
K226 ELVVHYKKGKDGLCQ
S236 DGLCQKLSVPCVSPK
K281 VWMATYNKHTKVAVK
K284 ATYNKHTKVAVKTMK
T309 AEANLMKTLQHDKLV
F328 VVSQEPIFIVTEFMA
Y409‑p RIIEDNEytAREGAK
T410‑p IIEDNEytAREGAKF
S440 TIKSDVWSFGILLME
S460 RIPYPGMSNPEVIRA
S518 DFYTATESQYQQQP_
Y520 YTATESQYQQQP___
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