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Protein Page:
BAD (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
BAD a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL. Note: This description may include information from UniProtKB.
Protein type: Apoptosis
Chromosomal Location of Human Ortholog: 11q13.1
Cellular Component: cytosol; mitochondrial outer membrane; mitochondrion
Molecular Function: caspase activator activity; lipid binding; phospholipid binding; protein binding; protein kinase binding
Biological Process: ADP metabolic process; apoptosis; ATP metabolic process; caspase activation; glucose homeostasis; negative regulation of cytolysis; pore complex biogenesis; positive regulation of apoptosis; positive regulation of autophagy; positive regulation of caspase activity; positive regulation of epithelial cell proliferation; positive regulation of glucokinase activity; positive regulation of insulin secretion; positive regulation of proteolysis; protein insertion into mitochondrial membrane during induction of apoptosis; regulation of mitochondrial membrane permeability
Reference #:  Q92934 (UniProtKB)
Alt. Names/Synonyms: BAD; BBC2; BBC6; Bcl-2-binding component 6; Bcl-2-like protein 8; BCL-X/BCL-2 binding protein; Bcl-XL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death; BCL2-antagonist of cell death protein; BCL2-associated agonist of cell death; BCL2-binding component 6; BCL2-binding protein; Bcl2-L-8; BCL2L8
Gene Symbols: BAD
Molecular weight: 18,392 Da
Basal Isoelectric point: 6.6  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  ErbB/HER Signaling  |  Growth And Differentiation Control by MAPKs  |  Inhibition of Apoptosis  |  Insulin Receptor Signaling  |  Mitochondrial Control of Apoptosis  |  PI3K/Akt Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

BAD

Protein Structure Not Found.
Download PyMol Script
Download ChimeraX Script


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Sites Implicated In
apoptosis, altered: S75‑p, S99‑p, S118‑p
apoptosis, inhibited: S75‑p, S99‑p, S118‑p, S124‑p, S134‑p
cell adhesion, altered: S75‑p
cell growth, altered: S75‑p, S99‑p
cell growth, induced: S134‑p
transcription, altered: S75‑p
transcription, inhibited: S75‑p, S99‑p
activity, induced: S75‑p, S99‑p, T137‑p
activity, inhibited: S75‑p
molecular association, regulation: S74‑p, S75‑p, R94‑m2, R96‑m2, S99‑p, S118‑p
phosphorylation: S74‑p, R94‑m2, R96‑m2, S99‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 - gap
1 0 - gap
0 9 S25‑p AERGLGPsPAGDGPs
0 2 S32‑p sPAGDGPsGsGkHHR
0 5 S34‑p AGDGPsGsGkHHRQA
0 1 K36‑ac DGPsGsGkHHRQAPG
1 0 S57 HQQEQPTSSSHHGGA
1 2 S59 QEQPTSSSHHGGAGA
0 30 S71‑p AGAVEIRsRHssyPA
2 80 S74‑p VEIRsRHssyPAGtE
119 188 S75‑p EIRsRHssyPAGtED
0 51 Y76‑p IRsRHssyPAGtEDD
1 32 T80‑p HssyPAGtEDDEGMG
7 45 S91‑p EGMGEEPsPFrGrsR
1 0 R94‑m2 GEEPsPFrGrsRsAP
1 0 R96‑m2 EPsPFrGrsRsAPPN
1 24 S97‑p PsPFrGrsRsAPPNL
117 257 S99‑p PFrGrsRsAPPNLWA
0 1 Y110‑p NLWAAQRyGRELRRM
45 211 S118‑p GRELRRMsDEFVDsF
1 4 S124‑p MsDEFVDsFKKGLPR
6 17 S134‑p KGLPRPKsAGtAtQM
2 0 T137‑p PRPKsAGtAtQMRQS
0 1 T139‑p PKsAGtAtQMRQSSs
0 1 S144 tAtQMRQSSsWTRVF
0 2 S146‑p tQMRQSSsWTRVFQS
0 5 R161‑m1 WWDRNLGrGSSAPSQ
3 0 A165 NLGrGSSAPSQ____
5193 : Phospho-Bad (Ser112) (7E11) Mouse mAb (Biotinylated)
5284 : Phospho-Bad (Ser112) (40A9) Rabbit mAb
9291 : Phospho-Bad (Ser112) Antibody
9296 : Phospho-Bad (Ser112) (7E11) Mouse mAb
11865 : Phospho-Bad (Ser112) (40A9) Rabbit mAb (PE Conjugate)
4366 : Phospho-Bad (Ser136) (D25H8) Rabbit mAb
5286 : Phospho-Bad (Ser136) (185D10) Rabbit mAb
9295 : Phospho-Bad (Ser136) Antibody
9297 : Phospho-Bad (Ser155) Antibody
  mouse

 
S20‑p HALGLRKsDPGIRsL
S26‑p KsDPGIRsLGSDAGG
S67 TDRGLGPSLTEDQPG
G74 SLTEDQPGPyLAPGL
Y76‑p TEDQPGPyLAPGLLG
- gap
T94‑p HQQGRAAtNsHHGGA
S96‑p QGRAAtNsHHGGAGA
S108‑p AGAMETRsRHssyPA
S111‑p METRsRHssyPAGtE
S112‑p ETRsRHssyPAGtEE
Y113‑p TRsRHssyPAGtEED
T117‑p HssyPAGtEEDEGME
S128‑p EGMEEELsPFRGRsR
R131 EEELsPFRGRsRsAP
R133 ELsPFRGRsRsAPPN
S134‑p LsPFRGRsRsAPPNL
S136‑p PFRGRsRsAPPNLWA
Y147 NLWAAQRYGRELRRM
S155‑p GRELRRMsDEFEGsF
S161‑p MsDEFEGsFKGLPRP
S170‑p KGLPRPKsAGtATQM
T173‑p PRPKsAGtATQMRQS
T175 PKsAGtATQMRQSAG
S180 tATQMRQSAGWTRII
G182 TQMRQSAGWTRIIQS
K197 WWDRNLGKGGStPSQ
T201‑p NLGKGGStPSQ____
5193 : Phospho-Bad (Ser112) (7E11) Mouse mAb (Biotinylated)
5284 : Phospho-Bad (Ser112) (40A9) Rabbit mAb
9291 : Phospho-Bad (Ser112) Antibody
9296 : Phospho-Bad (Ser112) (7E11) Mouse mAb
11865 : Phospho-Bad (Ser112) (40A9) Rabbit mAb (PE Conjugate)
4366 : Phospho-Bad (Ser136) (D25H8) Rabbit mAb
5286 : Phospho-Bad (Ser136) (185D10) Rabbit mAb
9295 : Phospho-Bad (Ser136) Antibody
9297 : Phospho-Bad (Ser155) Antibody
  rat

 
S20 HALGLRKSDPGIRSL
S26 KSDPGIRSLGSDAGG
S67 TDRGLGPSLTEDQPG
G74 SLTEDQPGPYLAPGL
Y76 TEDQPGPYLAPGLLG
- gap
N95 QQPGQAANNSHHGGA
S97 PGQAANNSHHGGAGT
S109 AGTMETRSRHSsYPA
S112 METRSRHSsYPAGtE
S113‑p ETRSRHSsYPAGtEE
Y114 TRSRHSsYPAGtEED
T118‑p HSsYPAGtEEDEGME
S129‑p EGMEEELsPFRGRsR
R132 EEELsPFRGRsRsAP
R134 ELsPFRGRsRsAPPN
S135‑p LsPFRGRsRsAPPNL
S137‑p PFRGRsRsAPPNLWA
Y148 NLWAAQRYGRELRRM
S156‑p GRELRRMsDEFEGSF
S162 MsDEFEGSFKGLPRP
S171 KGLPRPKSAGTATQM
T174 PRPKSAGTATQMRQs
T176 PKSAGTATQMRQsAs
S181‑p TATQMRQsAsWTRII
S183‑p TQMRQsAsWTRIIQS
K198 WWDRNLGKGGSTPSQ
T202 NLGKGGSTPSQ____
5193 : Phospho-Bad (Ser112) (7E11) Mouse mAb (Biotinylated)
5284 : Phospho-Bad (Ser112) (40A9) Rabbit mAb
9291 : Phospho-Bad (Ser112) Antibody
9296 : Phospho-Bad (Ser112) (7E11) Mouse mAb
11865 : Phospho-Bad (Ser112) (40A9) Rabbit mAb (PE Conjugate)
4366 : Phospho-Bad (Ser136) (D25H8) Rabbit mAb
5286 : Phospho-Bad (Ser136) (185D10) Rabbit mAb
9295 : Phospho-Bad (Ser136) Antibody
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