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Protein Page:
ATF-4 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
ATF-4 a transcription factor that is a member of the leucine zipper family. Isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). Involved in cisplatin resistance. Other members of the family include AP-1 transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. Two alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 22q13.1
Cellular Component: cytoplasm; neuron projection; nucleoplasm; nucleus
Molecular Function: DNA binding; leucine zipper domain binding; protein binding; sequence-specific DNA binding; transcription factor activity
Biological Process: amino acid metabolic process; cellular response to amino acid starvation; cellular response to glucose starvation; circadian regulation of gene expression; gluconeogenesis; mRNA transcription from RNA polymerase II promoter; negative regulation of translation initiation in response to stress; positive regulation of apoptosis; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase I promoter; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter
Reference #:  P18848 (UniProtKB)
Alt. Names/Synonyms: Activating transcription factor 4; activating transcription factor 4 (tax-responsive enhancer element B67); ATF4; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB-2; CREB2; Cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; Tax-responsive enhancer element-binding protein 67; TaxREB67; TXREB
Gene Symbols: ATF4
Molecular weight: 38,590 Da
Basal Isoelectric point: 4.83  Predict pI for various phosphorylation states
CST Pathways:  Growth And Differentiation Control by MAPKs
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

ATF-4

Protein Structure Not Found.
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Sites Implicated In
carcinogenesis, induced: S245‑p
cell growth, altered: S245‑p
transcription, altered: S219‑p
transcription, induced: S215‑p
transcription, inhibited: T107‑p, T114‑p, T115‑p, T119‑p
activity, induced: S245‑p
molecular association, regulation: S219‑p, S224‑p
protein degradation: T107‑p, T114‑p, T115‑p, T119‑p
protein stabilization: S215‑p, S219‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 K45‑ub LEVAKHFkPHGFSSD
0 1 K45‑sm LEVAKHFkPHGFSSD
0 1 K53‑sm PHGFSSDkAkAGSSE
0 1 K55‑ub GFSSDkAkAGSSEWL
0 3 S69‑p LAVDGLVsPSNNSkE
0 1 K75‑ub VsPSNNSkEDAFSGT
0 1 K88‑ub GTDWMLEkMDLKEFD
0 1 K92 MLEkMDLKEFDLDAL
1 1 T107‑p LGIDDLEtMPDDLLt
1 1 T114‑p tMPDDLLttLDDtCD
1 1 T115‑p MPDDLLttLDDtCDL
1 1 T119‑p LLttLDDtCDLFAPL
1 0 S215‑p IKEEDTPsDNDsGIC
3 0 S219‑p DTPsDNDsGICMsPE
1 0 S224‑p NDsGICMsPESYLGS
3 1 S245‑p TRGSPNRsLPsPGVL
0 2 S248‑p SPNRsLPsPGVLCGS
1 0 L252 sLPsPGVLCGSARPK
0 1 K267‑sm PYDPPGEkMVAAKVK
0 1 K277‑ub AAKVKGEkLDKKLKK
0 1 T293‑p EQNKTAAtRyRQKKR
0 1 Y295‑p NKTAAtRyRQKKRAE
0 1 K335‑ub AKEIQYLkDLIEEVR
0 6 K343‑ac DLIEEVRkARGKkRV
0 6 K348‑ac VRkARGKkRVP____
  mouse

 
K45 LEVAKHLKPHGFSSD
K45 LEVAKHLKPHGFSSD
K53 PHGFSSDKAGSSEWP
- gap
S68 AMDDGLASASDTGKE
K74 ASASDTGKEDAFSGT
K87 GTDWMLEKMDLkEFD
K91‑ub MLEKMDLkEFDFDAL
T106 FRMDDLETMPDELLT
T113 TMPDELLTTLDDTCD
T114 MPDELLTTLDDTCDL
T118 LLTTLDDTCDLFAPL
S214 VKEEDTPSDNDSGIC
S218 DTPSDNDSGICMSPE
S223 NDSGICMSPESYLGS
N244 TSRAPPDNLPSPGGs
S247 APPDNLPSPGGsRGS
S251‑p NLPSPGGsRGSPRPK
S266 PYDPPGVSLTAKVKT
K275 TAKVKTEKLDKKLKK
T291 EQNKTAATRYRQKKR
Y293 NKTAATRYRQKKRAE
K333 AKEIQYLKDLIEEVR
K341‑ac DLIEEVRkARGKkRV
K346‑ac VRkARGKkRVP____
  rat

 
K45 LEVAKHFKPHGFSSD
K45 LEVAKHFKPHGFSSD
K53 PHGFSSDKAGSSEWL
- gap
S67 LAMDGLVSASDTGKE
K73 VSASDTGKEDAFSGT
K86 GTDWMLEKMDLKEFD
K90 MLEKMDLKEFDFDAL
T105 FRMDDLETMPDELLA
A112 TMPDELLATLDDTCD
T113 MPDELLATLDDTCDL
T117 LLATLDDTCDLFAPL
S213 VKEEDTPSDSDSGIC
S217 DTPSDSDSGICMSPE
S222 SDSGICMSPESYLGS
S243 TSRAPPDSLPSPGVP
S246 APPDSLPSPGVPRGS
P250 SLPSPGVPRGSRPKP
S264 PYDPPGVSVTAKVKT
K273 TAKVKTEKLDKKLKK
T289 EQNKTAATRYRQKKR
Y291 NKTAATRYRQKKRAE
K331 AKEIQYLKDLIEEVR
K339 DLIEEVRKARGKKRV
K344 VRKARGKKRVP____
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