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Protein Page:
SMAD1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
SMAD1 transcription factor phosphorylated and activated by bone morphogenetic protein type 1 receptor kinases. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. Phosphorylated forms dimerize with collaborating Smad4 and are translocated into the nucleus, where the transcription of target genes is stimulated. Note: This description may include information from UniProtKB.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 4q31
Cellular Component: cytoplasm; cytosol; integral to membrane; intracellular; nuclear inner membrane; nucleoplasm; nucleus; protein complex; transcription factor complex
Molecular Function: identical protein binding; metal ion binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; receptor signaling protein activity; transcription factor activity; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity
Biological Process: BMP signaling pathway; cardiac muscle cell proliferation; cartilage development; embryonic pattern specification; gamete generation; hindbrain development; homeostatic process; inflammatory response; MAPKKK cascade; mesodermal cell fate commitment; midbrain development; negative regulation of cell proliferation; osteoblast fate commitment; positive regulation of osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; primary microRNA processing; signal transduction; SMAD protein complex assembly; transcription from RNA polymerase II promoter; transforming growth factor beta receptor signaling pathway; ureteric bud development
Reference #:  Q15797 (UniProtKB)
Gene Symbols: SMAD1
Molecular weight: 52,260 Da
Basal Isoelectric point: 6.9  Predict pI for various phosphorylation states
CST Pathways:  ESC Pluripotency and Differentiation  |  TGF-ß Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

SMAD1

Protein Structure Not Found.
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Sites Implicated In
transcription, altered: S206‑p, S462‑p, S463‑p, S465‑p
activity, induced: S462‑p, S463‑p, S465‑p
intracellular localization: S187‑p, S195‑p, S206‑p, S214‑p, S462‑p, S463‑p, S465‑p
molecular association, regulation: S206‑p, S214‑p, S462‑p, S463‑p, S465‑p
protein degradation: S206‑p
ubiquitination: S206‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 S11‑p TSLFSFTsPAVKRLL
1 0 K116‑ub CEFPFGSkQkEVCIN
1 0 K118‑ub FPFGSkQkEVCINPY
0 8 S132‑p YHYKRVEsPVLPPVL
0 1 S144‑p PVLVPRHsEyNPQHS
0 1 Y146‑p LVPRHsEyNPQHSLL
4 0 S187‑p NSHPFPHsPNSSYPN
3 0 S195‑p PNSSYPNsPGSSSSt
1 0 T202‑p sPGSSSStYPHsPTS
3 0 S206‑p SSStYPHsPTSSDPG
3 0 S214‑p PTSSDPGsPFQMPAD
1 0 S239‑p DPMTQDGsQPMDTNM
1 0 K269‑ub AVAYEEPkHWCSIVY
0 1 T300 SVLVDGFTDPSNNKN
0 1 S303 VDGFTDPSNNKNRFC
2 2 T322‑p SNVNRNStIENTRRH
0 1 S456‑p KVLTQMGsPHNPIss
2 8 S462‑p GsPHNPIssVs____
10 69 S463‑p sPHNPIssVs_____
10 76 S465‑p HNPIssVs_______
5753 : Phospho-Smad1 (Ser206) (D40B7) Rabbit mAb
9553 : Phospho-Smad1 (Ser206) Antibody
9511 : Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad8 (Ser426/428) Antibody
9516 : Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb
9576 : Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb (Biotinylated)
11971 : Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb (ChIP formulated)
12428 : Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb (PE Conjugate)
13820 : Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb
9511 : Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad8 (Ser426/428) Antibody
9516 : Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb
9576 : Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb (Biotinylated)
11971 : Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb (ChIP formulated)
12428 : Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb (PE Conjugate)
13820 : Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb
  mouse

 
S11 TSLFSFTSPAVKRLL
K116 CEFPFGSKQKEVCIN
K118 FPFGSKQKEVCINPY
S132‑p YHYKRVEsPVLPPVL
S144‑p PVLVPRHsEYNPQHS
Y146 LVPRHsEYNPQHSLL
S187 NSHPFPHSPNSSYPN
S195 PNSSYPNSPGGSSST
T202 SPGGSSSTYPHSPTS
S206 SSSTYPHSPTSSDPG
S214 PTSSDPGSPFQMPAD
S239 DPMAQDGSQPMDTNM
K269 AVAYEEPKHWCSIVY
T300 SVLVDGFTDPSNNKN
S303 VDGFTDPSNNKNRFC
T322‑p SNVNRNStIENTRRH
S456 KVLTQMGSPHNPIss
S462‑p GSPHNPIssVs____
S463‑p SPHNPIssVs_____
S465‑p HNPIssVs_______
5753 : Phospho-Smad1 (Ser206) (D40B7) Rabbit mAb
9553 : Phospho-Smad1 (Ser206) Antibody
9511 : Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad8 (Ser426/428) Antibody
9516 : Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb
9576 : Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb (Biotinylated)
11971 : Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb (ChIP formulated)
12428 : Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb (PE Conjugate)
13820 : Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb
9511 : Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad8 (Ser426/428) Antibody
9516 : Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb
9576 : Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb (Biotinylated)
11971 : Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb (ChIP formulated)
12428 : Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb (PE Conjugate)
13820 : Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb
  rat

 
S11 TSLFSFTSPAVKRLL
K116 CEFPFGSKQKEVCIN
K118 FPFGSKQKEVCINPY
S132 YHYKRVESPVLPPVL
S144 PVLVPRHSEYNPQHS
Y146 LVPRHSEYNPQHSLL
S187 HSHPFPQSPNSSYPN
S195 PNSSYPNSPGSSSST
T202 SPGSSSSTYPHSPTS
S206 SSSTYPHSPTSSDPG
S214 PTSSDPGSPFQMPAD
S239 DPMAQDGSQPMDTNM
K272 AVAYEEPKHWCSIVY
T303‑p SVLVDGFtDPsNNKN
S306‑p VDGFtDPsNNKNRFC
T325‑p SNVNRNStIENTRRH
S459 KVLTQMGSPHNPISs
S465 GSPHNPISsVs____
S466‑p SPHNPISsVs_____
S468‑p HNPISsVs_______
5753 : Phospho-Smad1 (Ser206) (D40B7) Rabbit mAb
9553 : Phospho-Smad1 (Ser206) Antibody
9511 : Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad8 (Ser426/428) Antibody
9516 : Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb
9576 : Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb (Biotinylated)
11971 : Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb (ChIP formulated)
12428 : Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb (PE Conjugate)
13820 : Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb
9511 : Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad8 (Ser426/428) Antibody
9516 : Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb
9576 : Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb (Biotinylated)
11971 : Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb (ChIP formulated)
12428 : Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb (PE Conjugate)
13820 : Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb
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