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Protein Page:
p21Cip1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
p21Cip1 a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. Its expression is tightly controlled by p53, through which this protein mediates the p53-dependent cell cycle arrest at G1 phase. Note: This description may include information from UniProtKB.
Protein type: Inhibitor; Cell cycle regulation
Chromosomal Location of Human Ortholog: 6p21.2
Cellular Component: cyclin-dependent protein kinase holoenzyme complex; cytosol; nucleoplasm; nucleus
Molecular Function: cyclin-dependent protein kinase activating kinase activity; cyclin-dependent protein kinase inhibitor activity; protein binding; ubiquitin protein ligase binding
Biological Process: cell cycle arrest; cellular response to amino acid starvation; cellular response to extracellular stimulus; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; G1/S transition of mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of phosphorylation; positive regulation of fibroblast proliferation; protein stabilization; Ras protein signal transduction; regulation of cyclin-dependent protein kinase activity; response to DNA damage stimulus
Reference #:  P38936 (UniProtKB)
Alt. Names/Synonyms: CAP20; CDK-interacting protein 1; CDK-interaction protein 1; CDKN1; CDKN1A; CDN1A; CIP1; Cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); DNA synthesis inhibitor; MDA-6; MDA6; melanoma differentiation associated protein 6; Melanoma differentiation-associated protein 6; p21; p21CIP1; p21Cip1/Waf1; PIC1; SDI1; WAF1; wild-type p53-activated fragment 1
Gene Symbols: CDKN1A
Molecular weight: 18,119 Da
Basal Isoelectric point: 8.69  Predict pI for various phosphorylation states
CST Pathways:  AMPK Signaling  |  G1/S Checkpoint  |  G2/M DNA Damage Checkpoint  |  PI3K/Akt Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

p21Cip1

Protein Structure Not Found.
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Sites Implicated In
cell cycle regulation: T57‑p, S130‑p, T145‑p
cell growth, altered: S146‑p
cell growth, inhibited: T145‑p
intracellular localization: T57‑p, S130‑p, T145‑p, S146‑p, S153‑p
molecular association, regulation: S130‑p, T145‑p, S146‑p, S153‑p
protein degradation: K16‑ub, T57‑p, K75‑ub, S114‑p, S130‑p, K141‑ub, K154‑ub, K161‑ub, K163‑ub
protein stabilization: T57‑p, S130‑p, T145‑p, S146‑p
ubiquitination: K16‑ub, T57‑p, K75‑ub, S130‑p, K141‑ub, K154‑ub, K161‑ub, K163‑ub

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S2 ______MSEPAGDVR
2 0 K16‑ub RQNPCGSkACRRLFG
0 1 S31‑p PVDSEQLsRDCDALM
7 0 T57‑p NFDFVTEtPLEGDFA
2 7 K75‑ub VRGLGLPkLYLPtGP
1 0 Y77 GLGLPkLYLPtGPRR
2 4 P79 GLPkLYLPtGPRRGR
1 0 T80‑p LPkLYLPtGPRRGRD
3 0 S98‑p GGRRPGTsPALLQGT
1 0 S114‑p EEDHVDLsLSCTLVP
2 0 S123‑p SCTLVPRsGEQAEGs
9 8 S130‑p sGEQAEGsPGGPGDS
1 0 K141‑ac PGDSQGRkRRQtsMT
2 0 K141‑ub PGDSQGRkRRQtsMT
17 0 T145‑p QGRkRRQtsMTDFyH
9 0 S146‑p GRkRRQtsMTDFyHs
0 1 Y151‑p QtsMTDFyHskRrLI
2 1 S153‑p sMTDFyHskRrLIFS
1 0 K154‑ac MTDFyHskRrLIFSk
2 0 K154‑ub MTDFyHskRrLIFSk
1 0 R156‑m2 DFyHskRrLIFSkRk
2 0 K161‑ac kRrLIFSkRkP____
2 0 K161‑ub kRrLIFSkRkP____
2 0 K163‑ac rLIFSkRkP______
2 0 K163‑ub rLIFSkRkP______
  mouse

 
S2‑p ______MsNPGDVRP
K15 RPVPHRSKVCRCLFG
R30 PVDSEQLRRDCDALM
T56 NFDFVTETPLEGNFV
K74 VRSLGLPKVyLsPGS
Y76‑p SLGLPKVyLsPGSRS
S78‑p GLPKVyLsPGSRSRD
P79 LPKVyLsPGSRSRDD
S96 GDKRPSTSSALLQGP
S112 PEDHVALSLSCTLVS
- gap
S125 VSERPEDSPGGPGTS
K136 PGTSQGRKRRQtsLT
K136 PGTSQGRKRRQtsLT
T140‑p QGRKRRQtsLTDFYH
S141‑p GRKRRQtsLTDFYHS
Y146 QtsLTDFYHSKRRLV
S148 sLTDFYHSKRRLVFC
K149 LTDFYHSKRRLVFCK
K149 LTDFYHSKRRLVFCK
R151 DFYHSKRRLVFCKRK
K156 KRRLVFCKRKP____
K156 KRRLVFCKRKP____
K158 RLVFCKRKP______
K158 RLVFCKRKP______
  dog

 
- gap
- gap
- gap
T25 NFDFVTETPLEGDFA
K43 VRGLGLSKVSLPAGP
S45 GLGLSKVSLPAGPRG
P47 GLSKVSLPAGPRGGR
A48 LSKVSLPAGPRGGRD
S66 GGKRPGTSPALLQGT
S82 QEDHLDLSLTCTLLP
S91‑p TCTLLPHsPERPEAS
S98 sPERPEASPGVPGTS
K109 PGTSQGRKRRQTSMT
K109 PGTSQGRKRRQTSMT
T113 QGRKRRQTSMTDFYH
S114 GRKRRQTSMTDFYHS
Y119 QTSMTDFYHSKRRLI
S121 SMTDFYHSKRRLIFS
K122 MTDFYHSKRRLIFSK
K122 MTDFYHSKRRLIFSK
R124 DFYHSKRRLIFSKRK
K129 KRRLIFSKRKP____
K129 KRRLIFSKRKP____
K131 RLIFSKRKP______
K131 RLIFSKRKP______
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