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Protein Page:
NFkB-p65 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
NFkB-p65 a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified. Note: This description may include information from UniProtKB.
Protein type: Nuclear receptor co-regulator; DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus; transcription factor complex
Molecular Function: actinin binding; chromatin binding; chromatin DNA binding; DNA binding; histone deacetylase binding; identical protein binding; NF-kappaB binding; phosphate binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein N-terminus binding; RNA polymerase II transcription factor activity, enhancer binding; transcription activator binding; transcription factor activity; transcription factor binding; ubiquitin protein ligase binding
Biological Process: activation of NF-kappaB transcription factor; cytokine and chemokine mediated signaling pathway; inflammatory response; membrane protein intracellular domain proteolysis; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of inflammatory response; response to organic substance; response to UV-B; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway
Reference #:  Q04206 (UniProtKB)
Alt. Names/Synonyms: MGC131774; NFKB3; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65; RELA; TF65; Transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)); v-rel reticuloendotheliosis viral oncogene homolog A (avian); v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65
Gene Symbols: RELA
Molecular weight: 60,219 Da
Basal Isoelectric point: 5.46  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  B Cell Receptor Signaling  |  Death Receptor Signaling  |  Inhibition of Apoptosis  |  NF-kB Signaling  |  Regulation of P38 MAPKs  |  T Cell Receptor Signaling  |  Toll-Like Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NFkB-p65

Protein Structure Not Found.
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Sites Implicated In
apoptosis, altered: S536‑p
apoptosis, inhibited: S276‑p, T505‑p, S536‑p
cell growth, altered: S536‑p
cell growth, inhibited: T505‑p
cell motility, altered: S536‑p
cell motility, inhibited: T505‑p
cytoskeletal reorganization: T505‑p
transcription, altered: K195‑ub, S205‑p, T254‑p, S276‑p, S281‑p, S468‑p, T505‑p, S529‑p, S536‑p
transcription, induced: S205‑p, T254‑p, S276‑p, S281‑p, S311‑p, S316‑p, T322‑gl, T352‑gl, S468‑p, S529‑p, S536‑p
transcription, inhibited: S276‑p, T435‑p, S468‑p, T505‑p
acetylation: S276‑p, S311‑p, S536‑p
activity, induced: T254‑p, S276‑p, S316‑p, S468‑p, S529‑p, S536‑p
activity, inhibited: K195‑ub, S468‑p
intracellular localization: S205‑p, T254‑p, S276‑p, S281‑p, S316‑p, S468‑p, S529‑p, S536‑p
molecular association, regulation: T254‑p, S276‑p, T322‑gl, T352‑gl, S536‑p
protein degradation: K195‑ub, S468‑p, S536‑p
protein stabilization: T254‑p, S276‑p, S536‑p
ubiquitination: S468‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 0 K37‑me RGMRFRYkCEGRsAG
1 0 K37‑m1 RGMRFRYkCEGRsAG
0 1 S42‑p RYkCEGRsAGsIPGE
0 4 S45‑p CEGRsAGsIPGERST
1 0 K56‑ub ERSTDTTkTHPTIkI
2 0 K62‑ub TkTHPTIkINGYTGP
0 1 T71‑p NGYTGPGtVRISLVT
1 0 K79‑ub VRISLVTkDPPHRPH
0 2 F99 GKDCRDGFYEAELCP
5 26 K122‑ac NLGIQCVkkRDLEQA
1 0 K122‑ub NLGIQCVkkRDLEQA
3 0 K123‑ac LGIQCVkkRDLEQAI
1 1 K123‑ub LGIQCVkkRDLEQAI
0 1 S131‑p RDLEQAIsQRIQTNN
1 0 R174‑m2 DPSGRPLrLPPVLSH
0 3 P176 SGRPLrLPPVLSHPI
2 0 K195‑ub APNTAELkICRVNRN
4 0 S205‑p RVNRNSGsCLGGDEI
5 0 K218‑ac EIFLLCDkVQkEDIE
1 0 K218‑me EIFLLCDkVQkEDIE
9 0 K221‑ac LLCDkVQkEDIEVYF
1 0 K221‑me LLCDkVQkEDIEVYF
0 4 S238‑p PGWEARGsFSQADVH
3 1 T254‑p QVAIVFRtPPYADPS
0 1 Y257 IVFRtPPYADPSLQA
66 0 S276‑p SMQLRRPsDRELsEP
5 0 S281‑p RPsDRELsEPMEFQY
0 1 T305‑p IEEKRKRtyEtFksI
1 0 T305‑gl IEEKRKRtyEtFksI
0 1 Y306‑p EEKRKRtyEtFksIM
0 1 T308‑p KRKRtyEtFksIMkk
44 2 K310‑ac KRtyEtFksIMkksP
1 0 K310‑m1 KRtyEtFksIMkksP
1 0 K310‑ub KRtyEtFksIMkksP
10 1 S311‑p RtyEtFksIMkksPF
3 0 K314‑ac EtFksIMkksPFSGP
2 0 K314‑m1 EtFksIMkksPFSGP
1 0 K314‑ub EtFksIMkksPFSGP
3 0 K315‑ac tFksIMkksPFSGPt
2 0 K315‑m1 tFksIMkksPFSGPt
1 0 K315‑ub tFksIMkksPFSGPt
1 3 S316‑p FksIMkksPFSGPtD
2 0 T322‑gl ksPFSGPtDPRPPPR
2 0 T352‑gl APQPYPFtSSLSTIN
2 0 T435‑p PTQAGEGtLSEALLQ
13 0 S468‑p AVFTDLAsVDNSEFQ
4 0 T505‑p EAITRLVtGAQRPPD
14 3 S529‑p GLPNGLLsGDEDFSs
125 2 S536‑p sGDEDFSsIADMDFS
0 1 S547‑p MDFSALLsQISS___
3045 : Acetyl-NF-kappaB p65 (Lys310) Antibody
12629 : Acetyl-NF-kappa-B p65 (Lys310) (D2S3J) Rabbit mAb
13188 : Methyl-NF-kB p65 (Lys310) Antibody
3039 : Phospho-NF-KappaB p65 (Ser468) Antibody
3031 : Phospho-NF-KappaB p65 (Ser536) Antibody
3033 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb
3036 : Phospho-NF-KappaB p65 (Ser536) (7F1) Mouse mAb
4025 : Phospho-NF-kB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated)
4886 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
4887 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
5733 : Phospho-NF-¿¿B p65 (Ser536) (93H1) Rabbit mAb (PE Conjugate)
13346 : Phospho-NF-¿B p65 (Ser536) (E1Z1T) Mouse mAb
  mouse

 
K37 RGMRFRYKCEGRSAG
K37 RGMRFRYKCEGRSAG
S42 RYKCEGRSAGsIPGE
S45‑p CEGRSAGsIPGERST
K56 ERSTDTTKTHPTIKI
K62 TKTHPTIKINGYTGP
T71 NGYTGPGTVRISLVT
K79 VRISLVTKDPPHRPH
Y99‑p GKDCRDGyYEADLCP
K122‑ac NLGIQCVkkRDLEQA
K122 NLGIQCVKkRDLEQA
K123‑ac LGIQCVkkRDLEQAI
K123‑ub LGIQCVkkRDLEQAI
S131 RDLEQAISQRIQTNN
L174 DPAGRPLLLtPVLSH
T176‑p AGRPLLLtPVLSHPI
K195 APNTAELKICRVNRN
S205 RVNRNSGSCLGGDEI
K218‑ac EIFLLCDkVQkEDIE
K218 EIFLLCDKVQkEDIE
K221‑ac LLCDkVQkEDIEVYF
K221 LLCDkVQKEDIEVYF
S238‑p PGWEARGsFSQADVH
T254 QVAIVFRTPPYADPS
Y257 IVFRTPPYADPSLQA
S276‑p SMQLRRPsDRELSEP
S281 RPsDRELSEPMEFQY
T305 IEEKRKRTYETFksI
T305 IEEKRKRTYETFksI
Y306 EEKRKRTYETFksIM
T308 KRKRTYETFksIMKK
K310‑ac KRTYETFksIMKKSP
K310 KRTYETFKsIMKKSP
K310 KRTYETFKsIMKKSP
S311‑p RTYETFksIMKKSPF
K314 ETFksIMKKSPFNGP
K314 ETFksIMKKSPFNGP
K314 ETFksIMKKSPFNGP
K315 TFksIMKKSPFNGPT
K315 TFksIMKKSPFNGPT
K315 TFksIMKKSPFNGPT
S316 FksIMKKSPFNGPTE
T322 KSPFNGPTEPRPPTR
P352 APQPYTFPASLSTIN
T433 STQAGEGTLSEALLH
S467‑p GVFTDLAsVDNSEFQ
T504‑p EAITRLVtGSQRPPD
S527 SGLPNGLSGDEDFSs
S534‑p SGDEDFSsIADMDFS
S545 MDFSALLSQISS___
3045 : Acetyl-NF-kappaB p65 (Lys310) Antibody
12629 : Acetyl-NF-kappa-B p65 (Lys310) (D2S3J) Rabbit mAb
13188 : Methyl-NF-kB p65 (Lys310) Antibody
3039 : Phospho-NF-KappaB p65 (Ser468) Antibody
3031 : Phospho-NF-KappaB p65 (Ser536) Antibody
3033 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb
3036 : Phospho-NF-KappaB p65 (Ser536) (7F1) Mouse mAb
4025 : Phospho-NF-kB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated)
4886 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
4887 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
5733 : Phospho-NF-¿¿B p65 (Ser536) (93H1) Rabbit mAb (PE Conjugate)
13346 : Phospho-NF-¿B p65 (Ser536) (E1Z1T) Mouse mAb
  rat

 
K37 RGMRFRYKCEGRSAG
K37 RGMRFRYKCEGRSAG
S42 RYKCEGRSAGSIPGE
S45 CEGRSAGSIPGERST
K56 ERSTDTTKTHPTIKI
K62 TKTHPTIKINGYTGP
T71 NGYTGPGTVRISLVT
K79 VRISLVTKDPPHRPH
F99 GKDCRDGFYEAELCP
K122 NLGIQCVKKRDLEQA
K122 NLGIQCVKKRDLEQA
K123 LGIQCVKKRDLEQAI
K123 LGIQCVKKRDLEQAI
S131 RDLEQAISQRIQTNN
R174 DPSGRPLRLTPVLSH
T176 SGRPLRLTPVLSHPI
K195 APNTAELKICRVNRN
S205 RVNRNSGSCLGGDEI
K218 EIFLLCDKVQKEDIE
K218 EIFLLCDKVQKEDIE
K221 LLCDKVQKEDIEVYF
K221 LLCDKVQKEDIEVYF
S238 PGWEARGSFSQADVH
T254‑p QVAIVFRtPPyADPS
Y257‑p IVFRtPPyADPSLQA
S276‑p SMQLRRPsDRELSEP
S281 RPsDRELSEPMEFQY
T305 IEEKRKRTYETFksI
T305 IEEKRKRTYETFksI
Y306 EEKRKRTYETFksIM
T308 KRKRTYETFksIMKK
K310‑ac KRTYETFksIMKKSP
K310 KRTYETFKsIMKKSP
K310 KRTYETFKsIMKKSP
S311‑p RTYETFksIMKKSPF
K314 ETFksIMKKSPFNGP
K314 ETFksIMKKSPFNGP
K314 ETFksIMKKSPFNGP
K315 TFksIMKKSPFNGPT
K315 TFksIMKKSPFNGPT
K315 TFksIMKKSPFNGPT
S316 FksIMKKSPFNGPTE
T322 KSPFNGPTEPRPPPR
S352 APQPYAFSTSLSTIN
T434 STQAGEGTLSEALLH
S468‑p GVFTDLAsVDNSEFQ
T505 EAITRLVTGSQRPPD
S528‑p SGLPNGLsGDEDFSs
S535‑p sGDEDFSsIADMDFS
S546 MDFSALLSQISS___
3045 : Acetyl-NF-kappaB p65 (Lys310) Antibody
13188 : Methyl-NF-kB p65 (Lys310) Antibody
3039 : Phospho-NF-KappaB p65 (Ser468) Antibody
3031 : Phospho-NF-KappaB p65 (Ser536) Antibody
3033 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb
3036 : Phospho-NF-KappaB p65 (Ser536) (7F1) Mouse mAb
4025 : Phospho-NF-kB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated)
4886 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
4887 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
5733 : Phospho-NF-¿¿B p65 (Ser536) (93H1) Rabbit mAb (PE Conjugate)
13346 : Phospho-NF-¿B p65 (Ser536) (E1Z1T) Mouse mAb
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