Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteprivacy & cookiesCuration ProcessContact
logos LINCs Logo Mt Sinai Logo NIH Logo NCI Logo
Protein Page:
MITF (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
MITF a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. Plays a critical role in the differentiation of various cell types including neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. Two isoforms are known: the M-isoform is expressed exclusively in melanocytes, while the A-isoform has a much broader range of expression. Mutations in MITF can lead to Waardenburg syndrome. Ten alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Oncoprotein; DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 3p14.2-p14.1
Cellular Component: nucleoplasm; protein complex
Molecular Function: protein binding
Biological Process: melanocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein complex assembly; protein sumoylation
Disease: Albinism, Ocular, With Sensorineural Deafness; Melanoma, Cutaneous Malignant, Susceptibility To, 8; Tietz Syndrome; Waardenburg Syndrome, Type 2a
Reference #:  O75030 (UniProtKB)
Alt. Names/Synonyms: BHLHE32; Class E basic helix-loop-helix protein 32; homolog of mouse microphthalmia; MI; Microphthalmia-associated transcription factor; microphthalmia-associated transcription factor-M transcript; MITF; WS2A
Gene Symbols: MITF
Molecular weight: 58,795 Da
Basal Isoelectric point: 5.93  Predict pI for various phosphorylation states
CST Pathways:  Growth And Differentiation Control by MAPKs
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

MITF

Protein Structure Not Found.
Download PyMol Script
Download ChimeraX Script


STRING  |  cBioPortal  |  Wikipedia  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Scansite  |  Pfam  |  RCSB PDB  |  Phospho.ELM  |  NetworKIN  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene


Sites Implicated In
protein degradation: S180‑p
ubiquitination: S180‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 - gap
0 1 K45 AEHPGASKPPISSSS
0 1 T54‑p PISSSSMtsRILLRQ
0 1 S55‑p ISSSSMtsRILLRQQ
0 1 K79‑sm ERREQQQkLQAAQFM
0 1 K140‑sm QAQRQQVkQYLSTTL
0 1 S157‑p KHANQVLsLPCPNQP
16 0 S180‑p PGSSAPNsPMAMLTL
0 1 S216‑p CPGMNTHsRASCMQM
1 0 S280 PGLTISNSCPANLPN
3 0 K289 PANLPNIKRELTACI
1 0 K308‑ub SEARALAkERQKKDN
0 1 S357‑p KGTILKAsVDyIRKL
0 1 Y360‑p ILKAsVDyIRKLQRE
3 1 S405‑p QARAHGLsLIPSTGL
2 23 S414‑p IPSTGLCsPDLVNRI
3 0 K423 DLVNRIIKQEPVLEN
0 2 T489‑p EDILMDDtLsPVGVT
0 14 S491‑p ILMDDtLsPVGVTDP
0 1 S502‑p VTDPLLSsVsPGAsK
1 10 S504‑p DPLLSsVsPGAsKts
1 2 S508‑p SsVsPGAsKtssRRs
0 1 T510‑p VsPGAsKtssRRssM
0 1 S511‑p sPGAsKtssRRssMS
1 1 S512‑p PGAsKtssRRssMSM
0 1 S515‑p sKtssRRssMSMEET
8 4 S516‑p KtssRRssMSMEETE
0 1 S518 ssRRssMSMEETEHT
  MITF iso5  
S32‑p KLTLCLFsRSSSAEH
K44 AEHPGASKPPISSSS
T53 PISSSSMTSRILLRQ
S54 ISSSSMTSRILLRQQ
K78 ERREQQQKLQAAQFM
K139 QAQRQQVKQYLSTTL
S156 KHANQVLSLPCPNQP
S179 PGSSAPNSPMAMLTL
S215 CPGMNTHSRASCMQM
S279 PGLTISNSCPANLPN
K288 PANLPNIKRELTACI
K307 SEARALAKERQKKDN
S356 KGTILKASVDYIRKL
Y359 ILKASVDYIRKLQRE
S404 QARAHGLSLIPSTGL
S413 IPSTGLCSPDLVNRI
K422 DLVNRIIKQEPVLEN
T488 EDILMDDTLSPVGVT
S490 ILMDDTLSPVGVTDP
S501 VTDPLLSSVSPGASK
S503 DPLLSSVSPGASKTS
S507 SSVSPGASKTSSRRS
T509 VSPGASKTSSRRSSM
S510 SPGASKTSSRRSSMS
S511 PGASKTSSRRSSMSM
S514 SKTSSRRSSMSMEET
S515 KTSSRRSSMSMEETE
S517 SSRRSSMSMEETEHT
  MITF iso9  
- gap
- gap
- gap
- gap
- gap
K33 QAQRQQVKQYLSTTL
S50 KHANQVLSLPCPNQP
S73‑p PGSSAPNsPMAMLTL
S109 CPGMNTHSRASCMQM
S173 PGLTISNSCPANLPN
K182‑sm PANLPNIkRELTACI
K201 SEARALAKERQKKDN
S250 KGTILKASVDYIRKL
Y253 ILKASVDYIRKLQRE
S298‑p QARAHGLsLIPSTGL
S307 IPSTGLCSPDLVNRI
K316‑sm DLVNRIIkQEPVLEN
T382 EDILMDDTLSPVGVT
S384 ILMDDTLSPVGVTDP
S395 VTDPLLSSVsPGAsK
S397‑p DPLLSSVsPGAsKTS
S401‑p SSVsPGAsKTSsRRS
T403 VsPGAsKTSsRRSsM
S404 sPGAsKTSsRRSsMS
S405‑p PGAsKTSsRRSsMSM
S408 sKTSsRRSsMSMEET
S409‑p KTSsRRSsMSMEETE
S411 SsRRSsMSMEETEHT
  mouse

► Hide Isoforms
 
- gap
K45 AEHPGASKPPISSSS
T54 PISSSSMTSRILLRQ
S55 ISSSSMTSRILLRQQ
K79 ERREQQQKLQAAQFM
K140 QAQRHQVKQYLSTTL
S157 KHASQVLSSPCPNQP
S180‑p PGSSAPNsPMAMLTL
S216 CPGMNTHSRASCMQM
S280 PGLTISNSCPANLPN
K289‑sm PANLPNIkRELTACI
K308 SEARALAKERQKKDN
S357‑p KGTILKAsVDyIRKL
Y360‑p ILKAsVDyIRKLQRE
S405‑p QARAHGLsLIPSTGL
S414‑p IPSTGLCsPDLVNRI
K423‑sm DLVNRIIkQEPVLEN
A489 EDILMDDALsPVGVT
S491‑p ILMDDALsPVGVTDP
S502 VTDPLLSSVsPGAsK
S504‑p DPLLSSVsPGAsKTS
S508‑p SSVsPGAsKTSSRRS
T510 VsPGAsKTSSRRSsM
S511 sPGAsKTSSRRSsMs
S512 PGAsKTSSRRSsMsA
S515 sKTSSRRSsMsAEET
S516‑p KTSSRRSsMsAEETE
S518‑p SSRRSsMsAEETEHA
  MITF iso8  
- gap
- gap
- gap
- gap
- gap
K33 QAQRHQVKQYLSTTL
S50 KHASQVLSSPCPNQP
S73‑p PGSSAPNsPMAMLTL
S109 CPGMNTHSRASCMQM
S173‑p PGLTISNsCPANLPN
K182‑sm PANLPNIkRELTACI
K201 SEARALAKERQKKDN
S250 KGTILKASVDYIRKL
Y253 ILKASVDYIRKLQRE
S298 QARAHGLSLIPSTGL
S307‑p IPSTGLCsPDLVNRI
K316‑sm DLVNRIIkQEPVLEN
A382 EDILMDDALSPVGVT
S384 ILMDDALSPVGVTDP
S395 VTDPLLSSVSPGASK
S397 DPLLSSVSPGASKTS
S401 SSVSPGASKTSSRRS
T403 VSPGASKTSSRRSsM
S404 SPGASKTSSRRSsMS
S405 PGASKTSSRRSsMSA
S408 SKTSSRRSsMSAEET
S409‑p KTSSRRSsMSAEETE
S411 SSRRSsMSAEETEHA
  rat

 
- gap
K45‑ac AEHSGASkPPLSSST
T54 PLSSSTMTSRILLRQ
S55 LSSSTMTSRILLRQQ
K79 ERREQQQKLQAAQFM
K140 QAQRHQVKQYLSTTL
S157 KHAGQVLSPPCPNQP
S180 PGSSAPNSPMAMLTL
S216 CPGMNTHSRASCMQM
S280 PGLTISNSCPANLPN
K289 PANLPNIKRELTACI
K308 SEARALAKERQKKDN
S357 KGTILKASVDYIRKL
Y360 ILKASVDYIRKLQRE
S405 QARAHGLSLIPSTGL
S414 IPSTGLCSPDLVNRI
K423 DLVNRIIKQEPVLEN
A489 EDILMDDALSPVGVT
S491 ILMDDALSPVGVTDP
S502 VTDPLLSSVSPGASK
S504 DPLLSSVSPGASKTS
S508 SSVSPGASKTSSRRS
T510 VSPGASKTSSRRSSM
S511 SPGASKTSSRRSSMS
S512 PGASKTSSRRSSMSA
S515 SKTSSRRSSMSAEET
S516 KTSSRRSSMSAEETE
S518 SSRRSSMSAEETEHA
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.