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PhosphoSitePlus

A Protein Modification Resource

PhosphoSitePlus^® (PSP) is an online systems biology resource providing comprehensive information and tools for the study of protein post-translational modifications (PTMs). In addition to providing an extensive, manually curated phosphorylation site database, other commonly studied PTMs are included in PSP. See About PhosphoSite above for more information.

Protein Sequences

Akt 3-D Representation

PROTEIN OR SUBSTRATE SEARCH

ADVANCED SEARCH AND BROWSE OPTIONS

	Protein, Sequence, or Reference Search Protein, Sequence, or Reference Search: Protein Searches retrieve lists of proteins and their modification types based on protein name or ID, protein type, domain, cellular component, and MW range. Sequence searches retrieve lists of proteins and sequences containing specified sequences, degenerate motifs, and domains. Reference searches retrieve lists of literature references specified by authors, proteins, or PubMedIDs.
	Site Search Site Search: Retrieves a list of modified sites (with surrounding amino-acid sequence) and proteins from which they come. Searches can be restricted by eight criteria: sites responsive to specific treatments, or those observed in specific protein types, domains, cellular components, disease states, cell lines, cell types, or tissues.
	Comparative Site Search Comparative Site Search: Retrieves a list of modified sites that possess certain specified attributes and exclude others. Searches can be restricted by the same eight properties as for the "Site Search".
	Browse MS2 Data By Disease Browse MS2 Data By Disease: Allows the user to browse curated MS/MS records by selecting a disease type. Results show the number of records and associated modification sites observed in the selected disease.
	Browse MS2 Data by Cell Line Browse MS2 Data by Cell Line: Allows the user to browse curated MS/MS records by selecting a cell line. Results show the number of records and associated modification sites observed in the selected cell line.
	Browse MS2 Data by Tissue Browse MS2 Data by Tissue: Allows the user to browse curated MS/MS records by selecting a tissue. Results show the number of records and associated modification sites observed in the selected tissue.

DOWNLOADS, LINKS & APPLICATIONS

	Reprints, References, Supplemental Tables
	Downloadable Datasets
	Motif Analysis Tools Browse MS2 Data by Cell Line: Allows the user to browse curated MS/MS records by selecting a cell line. Results show the number of records and associated modification sites observed in the selected cell line.

WHAT'S NEW

Dec 2011 Download "PhosphoSitePlus: a comprehensive resource…" in January 2012 issue of Nucleic Acids Research.

Jul 2011 Multiple Sequence Alignment (MSA) added to the Protein Page.

Jul 2011 Download PyMOL & Chimera Scripts from the Structure Viewer window.

Jul 2011 New Tutorial for Navigating PhosphoSitePlus^®.

Apr 2011 New Icon indicates links to protein-specific reagents from CST™

Mar 2011 Download breast cancer dataset associated with “Molecular Driver and Tumor Suppressor for Triple-Negative Breast Cancer”, Cell 144:Mar 4, 2011.

Mar 2011 Sequence logos of kinase substrates added to protein pages of kinases with 15 or more known targets (example: Akt1).

Oct 2010 Open PSPlogo, an interactive logo-generating tool to explore the profiles of modification sites observed in specific subsets of proteins.

Aug 2010 Download reprint “Akt-RSK-S6-kinase Signaling Networks Activated by Oncogenic Receptor Tyrosine Kinases”, Sci Signal. 3(136):Aug 24, 2010.

Phosphorylation Site Statistics
Non-redundant sites:	170,293
Non-redundant proteins:	18,884
Sites curated from literature:	102,680
All sites using site-specific (SS) methods:	11,174
All sites using discovery-mode MS (MS) methods:	94,009
Sites using both SS and MS methods:	5,364
MS sites observed at CST:	102,554
Number of curated papers:	13,898

Other Modification Site Statistics
Acetylation:	23,989	Caspase cleavage:	481
Di-methylation:	1,698	Methylation:	139
Mono-methylation:	3,456	O-GlcNAc:	604
Sumoylation:	681	Tri-methylation:	239
Ubiquitination:	39,502

Creative Commons License

PhosphoSite, created by Cell Signaling Technology is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. Information about permissions beyond the scope of this license are available at http://www.phosphosite.org/staticContact.do.

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