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A Protein Modification Resource

Protein Sequences

PhosphoSitePlus® (PSP) is an online systems biology resource providing comprehensive information and tools for the study of protein post-translational modifications (PTMs) including phosphorylation, ubiquitination, acetylation and methylation. See About PhosphoSite above for more information.

Please cite the following reference for this resource: Hornbeck PV. et al. (2012) Nucleic Acids Res. 2012 40:D261-70. [reprint]

Akt 3-D Representation
Protein and Sequence SearchProtein, Sequence, or Reference Search
Site Search
Comparative Site Search
Browse By DiseaseBrowse MS2 Data By Disease
Browse Cell LineBrowse MS2 Data by Cell Line
Browse by TissueBrowse MS2 Data by Tissue
Reprints, References, Supplemental Tables
Downloadable Datasets
Motif Analysis Tools
Aug 2014   Download PTM-VarMut dataset: Overlap of disease missense mutations & genetic variants, with their corresponding PTMs and flanking sequences.
Jul 2012   Download Datasets of Regulatory or Disease-Associated Sites.
Dec 2011   Download "PhosphoSitePlus: a comprehensive resource‚Ķ" in January 2012 issue of Nucleic Acids Research.
Jul 2011   Multiple Sequence Alignment (MSA) added to the Protein Page.
Jul 2011   Download PyMOL & Chimera Scripts from the Structure Viewer window.
Jul 2011   New Tutorial for Navigating PhosphoSitePlus®.
Apr 2011   New Icon indicates links to protein-specific reagents from CST™ Modification Specific Proteins
Mar 2011   Download breast cancer dataset associated with “Molecular Driver and Tumor Suppressor for Triple-Negative Breast Cancer”, Cell 144:Mar 4, 2011.
Mar 2011   Sequence logos of kinase substrates added to protein pages of kinases with 15 or more known targets (example: Akt1).
Oct 2010   Open PSPlogo, an interactive logo-generating tool to explore the profiles of modification sites observed in specific subsets of proteins.
Aug 2010   Download reprint “Akt-RSK-S6-kinase Signaling Networks Activated by Oncogenic Receptor Tyrosine Kinases”, Sci Signal. 3(136):Aug 24, 2010.
Phosphorylation Site Statistics
Non-redundant sites: 246,713
Non-redundant proteins: 19,717
Sites curated from literature: 145,003
All sites using site-specific (SS) methods: 12,684
All sites using discovery-mode MS (MS) methods: 136,161
Sites using both SS and MS methods: 6,246
MS sites observed at CST: 151,473
Number of curated papers: 16,491
Other Modification Site Statistics
Acetylation: 27,657 Caspase cleavage: 481
Di-methylation: 2,556 Methylation: 163
Mono-methylation: 4,995 O-GalNAc: 2,118
O-GlcNAc: 1,393 Succinylation: 4,657
Sumoylation: 816 Tri-methylation: 321
Ubiquitination: 51,257
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