Curated Information
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Curated Information Page
PubMed Id: 19665974 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Rinehart J, et al. (2009) Sites of regulated phosphorylation that control K-Cl cotransporter activity. Cell 138, 525-36 19665974
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T929-p - KCC2 (human)
Orthologous residues
KCC2 (human): T929‑p, KCC2 (mouse): T929‑p, KCC2 iso2 (mouse): T906‑p, KCC2 (rat): T929‑p, KCC2 iso2 (rat): T906‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  activity, inhibited

T1030-p - KCC2 (human)
Orthologous residues
KCC2 (human): T1030‑p, KCC2 (mouse): T1029‑p, KCC2 iso2 (mouse): T1006‑p, KCC2 (rat): T1030‑p, KCC2 iso2 (rat): T1007‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  activity, inhibited

S32-p - KCC3 (human)
Orthologous residues
KCC3 (human): S32‑p, KCC3 iso2 (human): , KCC3 (mouse): S32‑p, KCC3 iso2 (mouse): , KCC3 (rat): S32‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

T991-p - KCC3 (human)
Orthologous residues
KCC3 (human): T991‑p, KCC3 iso2 (human): T940‑p, KCC3 (mouse): T991‑p, KCC3 iso2 (mouse): T940‑p, KCC3 (rat): T991‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), erythrocyte-blood
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP2CA (human)
PHOSPHATASE PPP1CA (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE WNK1 (human) siRNA inhibition of enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypotonic buffer decrease
Downstream Regulation
 Effect of modification (function):  activity, inhibited

S1023-p - KCC3 (human)
Orthologous residues
KCC3 (human): S1023‑p, KCC3 iso2 (human): S972‑p, KCC3 (mouse): S1023‑p, KCC3 iso2 (mouse): S972‑p, KCC3 (rat): S1023‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S1029-p - KCC3 (human)
Orthologous residues
KCC3 (human): S1029‑p, KCC3 iso2 (human): S978‑p, KCC3 (mouse): S1029‑p, KCC3 iso2 (mouse): S978‑p, KCC3 (rat): S1029‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

T1048-p - KCC3 (human)
Orthologous residues
KCC3 (human): T1048‑p, KCC3 iso2 (human): T997‑p, KCC3 (mouse): T1048‑p, KCC3 iso2 (mouse): T997‑p, KCC3 (rat): T1048‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), erythrocyte-blood
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP2CA (human)
PHOSPHATASE PPP1CA (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE WNK1 (human) siRNA inhibition of enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypotonic buffer decrease
Downstream Regulation
 Effect of modification (function):  activity, inhibited

T929-p - KCC2 (mouse)
Orthologous residues
KCC2 (human): T929‑p, KCC2 (mouse): T929‑p, KCC2 iso2 (mouse): T906‑p, KCC2 (rat): T929‑p, KCC2 iso2 (rat): T906‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  'brain, embryonic'
 Cellular systems studied:  tissue
 Species studied:  mouse

T1029-p - KCC2 (mouse)
Orthologous residues
KCC2 (human): T1030‑p, KCC2 (mouse): T1029‑p, KCC2 iso2 (mouse): T1006‑p, KCC2 (rat): T1030‑p, KCC2 iso2 (rat): T1007‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  'brain, embryonic'
 Cellular systems studied:  tissue
 Species studied:  mouse


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