Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Curated Information Page
PubMed Id: 19767751 
Kosako H, et al. (2009) Phosphoproteomics reveals new ERK MAP kinase targets and links ERK to nucleoporin-mediated nuclear transport. Nat Struct Mol Biol 16, 1026-35 19767751
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

S257-p - NUP153 (human)
Orthologous residues
NUP153 (human): S257‑p, NUP153 (mouse): S258‑p, NUP153 (rat): S258‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK1 (human)
KINASE ERK2 (human)

S320-p - NUP153 (human)
Orthologous residues
NUP153 (human): S320‑p, NUP153 (mouse): S321‑p, NUP153 (rat): S321‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
KINASE ERK1 (human)

S334-p - NUP153 (human)
Orthologous residues
NUP153 (human): S334‑p, NUP153 (mouse): S335‑p, NUP153 (rat): S335‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
KINASE ERK1 (human)

S338-p - NUP153 (human)
Orthologous residues
NUP153 (human): S338‑p, NUP153 (mouse): S339‑p, NUP153 (rat): S339‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
KINASE ERK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
U0126 phorbol ester inhibit treatment-induced increase

T369-p - NUP153 (human)
Orthologous residues
NUP153 (human): T369‑p, NUP153 (mouse): T370‑p, NUP153 (rat): T370‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
KINASE ERK1 (human)

T388-p - NUP153 (human)
Orthologous residues
NUP153 (human): T388‑p, NUP153 (mouse): T389‑p, NUP153 (rat): T389‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK1 (human)
KINASE ERK2 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK1 (human) pharmacological inhibitor of upstream enzyme
KINASE ERK2 (human) pharmacological inhibitor of upstream enzyme
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
KPNB1 (human) Disrupts intracellular localization pull-down assay

T413-p - NUP153 (human)
Orthologous residues
NUP153 (human): T413‑p, NUP153 (mouse): S414‑p, NUP153 (rat): S414‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK1 (human)
KINASE ERK2 (human)

S516-p - NUP153 (human)
Orthologous residues
NUP153 (human): S516‑p, NUP153 (mouse): S514‑p, NUP153 (rat): S516‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK1 (human)
KINASE ERK2 (human)

S522-p - NUP153 (human)
Orthologous residues
NUP153 (human): S522‑p, NUP153 (mouse): S520‑p, NUP153 (rat): N522‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
KINASE ERK1 (human)

S529-p - NUP153 (human)
Orthologous residues
NUP153 (human): S529‑p, NUP153 (mouse): S527‑p, NUP153 (rat): S529‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK1 (human)
KINASE ERK2 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK2 (human) pharmacological inhibitor of upstream enzyme
KINASE ERK1 (human) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
U0126 phorbol ester inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
KPNB1 (human) Disrupts intracellular localization pull-down assay

S1710-p - NUP214 (human)
Orthologous residues
NUP214 (human): S1710‑p, NUP214 iso3 (human): S1711‑p, NUP214 iso4 (human): S1700‑p, NUP214 (mouse): S1706‑p, NUP214 (rat): S1689‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
KINASE ERK1 (human)

S1809-p - NUP214 (human)
Orthologous residues
NUP214 (human): S1809‑p, NUP214 iso3 (human): S1810‑p, NUP214 iso4 (human): S1799‑p, NUP214 (mouse): S1805‑p, NUP214 (rat): S1788‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK1 (human)
KINASE ERK2 (human)

S221-p - NUP50 (human)
Orthologous residues
NUP50 (human): S221‑p, NUP50 (mouse): P221‑p, NUP50 (rat): P222‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
KINASE CDK1 (human)
KINASE ERK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK2 (human) pharmacological inhibitor of upstream enzyme
KINASE CDK1 (human) pharmacological inhibitor of upstream enzyme
KINASE ERK1 (human) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
U0126 EGF inhibit treatment-induced increase
phorbol ester increase
U0126 phorbol ester inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
KPNB1 (human) Disrupts intracellular localization pull-down assay

S315-p - NUP50 (human)
Orthologous residues
NUP50 (human): S315‑p, NUP50 (mouse): S313‑p, NUP50 (rat): S314‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK1 (human)
KINASE ERK2 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK2 (human) pharmacological inhibitor of upstream enzyme
KINASE ERK1 (human) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
U0126 EGF inhibit treatment-induced increase
phorbol ester increase
U0126 phorbol ester inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
KPNB1 (human) Disrupts intracellular localization pull-down assay


Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.