Curated Information
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Curated Information Page
PubMed Id: 19667065 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Polzien L, et al. (2009) Identification of novel in vivo phosphorylation sites of the human proapoptotic protein BAD: pore-forming activity of BAD is regulated by phosphorylation. J Biol Chem 284, 28004-20 19667065
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

S25-p - BAD (human)
Orthologous residues
BAD (human): S25‑p, BAD (mouse): S67‑p, BAD (rat): S67‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  SF9
 Cellular systems studied:  cell lines
 Species studied:  fruit fly

S32-p - BAD (human)
Orthologous residues
BAD (human): S32‑p, BAD (mouse): G74‑p, BAD (rat): G74‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  SF9
 Cellular systems studied:  cell lines
 Species studied:  fruit fly

S34-p - BAD (human)
Orthologous residues
BAD (human): S34‑p, BAD (mouse): Y76‑p, BAD (rat): Y76‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  SF9
 Cellular systems studied:  cell lines
 Species studied:  fruit fly

S74-p - BAD (human)
Orthologous residues
BAD (human): S74‑p, BAD (mouse): S111‑p, BAD (rat): S112‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  SF9
 Cellular systems studied:  cell lines
 Species studied:  fruit fly

S75-p - BAD (human)
Orthologous residues
BAD (human): S75‑p, BAD (mouse): S112‑p, BAD (rat): S113‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast), SF9
 Cellular systems studied:  cell lines
 Species studied:  fruit fly, human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE BRAF (human)
KINASE RAF1 (human)
KINASE ARAF (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKACA (human) pharmacological activator of upstream enzyme, pharmacological inhibitor of upstream enzyme
KINASE RAF1 (human) transfection of wild-type enzyme
KINASE BRAF (human) transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IBMX, forskolin increase
H-89 forskolin, IBMX inhibit treatment-induced increase
PD98059 no change compared to control
U0126 no change compared to control
CI1040 no change compared to control
wortmannin no change compared to control
suramin no change compared to control
Bay 43-9006 decrease

S91-p - BAD (human)
Orthologous residues
BAD (human): S91‑p, BAD (mouse): S128‑p, BAD (rat): S129‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  SF9
 Cellular systems studied:  cell lines
 Species studied:  fruit fly

S97-p - BAD (human)
Orthologous residues
BAD (human): S97‑p, BAD (mouse): S134‑p, BAD (rat): S135‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  SF9
 Cellular systems studied:  cell lines
 Species studied:  fruit fly

S99-p - BAD (human)
Orthologous residues
BAD (human): S99‑p, BAD (mouse): S136‑p, BAD (rat): S137‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast), SF9
 Cellular systems studied:  cell lines
 Species studied:  fruit fly, human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PAK1 (human)
KINASE Akt1 (human)
KINASE ARAF (human)
KINASE BRAF (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE BRAF (human) transfection of wild-type enzyme
KINASE RAF1 (human) transfection of wild-type enzyme
KINASE Akt1 (human) transfection of constitutively active enzyme
KINASE PAK1 (human) transfection of constitutively active enzyme
KINASE PKACA (human) pharmacological activator of upstream enzyme, pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IBMX, forskolin increase
H-89 forskolin, IBMX inhibit treatment-induced increase
PD98059 no change compared to control
U0126 no change compared to control
CI1040 no change compared to control
wortmannin no change compared to control
suramin no change compared to control
Bay 43-9006 decrease
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 zeta (human) Induces in vitro

S118-p - BAD (human)
Orthologous residues
BAD (human): S118‑p, BAD (mouse): S155‑p, BAD (rat): S156‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast), SF9
 Cellular systems studied:  cell lines
 Species studied:  fruit fly, human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE BRAF (human)
KINASE ARAF (human)
KINASE RAF1 (human)
KINASE PAK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE RAF1 (human) transfection of wild-type enzyme
KINASE PAK1 (human) transfection of constitutively active enzyme
KINASE PKACA (human) pharmacological activator of upstream enzyme, pharmacological inhibitor of upstream enzyme
KINASE BRAF (human) transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IBMX, forskolin increase
H-89 forskolin, IBMX inhibit treatment-induced increase
PD98059 no change compared to control
U0126 no change compared to control
CI1040 no change compared to control
wortmannin no change compared to control
suramin no change compared to control
Bay 43-9006 decrease

S124-p - BAD (human)
Orthologous residues
BAD (human): S124‑p, BAD (mouse): S161‑p, BAD (rat): S162‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  SF9
 Cellular systems studied:  cell lines
 Species studied:  fruit fly

S134-p - BAD (human)
Orthologous residues
BAD (human): S134‑p, BAD (mouse): S170‑p, BAD (rat): S171‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  SF9
 Cellular systems studied:  cell lines
 Species studied:  fruit fly


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