Curated Information
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Curated Information Page
PubMed Id: 17928407 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Komatsu S, Ikebe M (2007) The Phosphorylation of Myosin II at the Ser1 and Ser2 Is Critical for Normal Platelet-derived Growth Factor induced Reorganization of Myosin Filaments. Mol Biol Cell 18, 5081-90 17928407
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S2-p - MRLC2 (mouse) ▲
Orthologous residues
MRLC2 (human): S2‑p, MRLC2 (mouse): S2‑p, MRLC2 (rat): S2‑p, MRLC2 (cow): S2‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE PKCA (cow)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
GF109203X PDGF inhibit treatment-induced increase
LY294002 PDGF no effect upon treatment-induced increase
PD98059 PDGF no effect upon treatment-induced increase
SB203580 PDGF no effect upon treatment-induced increase
Go 6976 PDGF inhibit treatment-induced increase
rottlerin PDGF no effect upon treatment-induced increase
Downstream Regulation
 Effect of modification (process):  cell adhesion, altered, cytoskeletal reorganization
 Comments:  induced disassembly of stress fibers and decreased large focal adhesions

S3-p - MRLC2 (mouse) ▲
Orthologous residues
MRLC2 (human): S3‑p, MRLC2 (mouse): S3‑p, MRLC2 (rat): S3‑p, MRLC2 (cow): S3‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
Downstream Regulation
 Effect of modification (process):  cell adhesion, altered, cytoskeletal reorganization
 Comments:  induced disassembly of stress fibers and decreased large focal adhesions

T19-p - MRLC2 (mouse) ▲
Orthologous residues
MRLC2 (human): T19‑p, MRLC2 (mouse): T19‑p, MRLC2 (rat): T19‑p, MRLC2 (cow): T19‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase

S20-p - MRLC2 (mouse) ▲
Orthologous residues
MRLC2 (human): S20‑p, MRLC2 (mouse): S20‑p, MRLC2 (rat): S20‑p, MRLC2 (cow): S20‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase


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