|
Orthologous residues
|
|
Mad1 (human): S145‑p, Mad1 (mouse): S144‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
|
|
Relevant cell lines - cell types - tissues:
293 (epithelial)
|
|
Cellular systems studied:
cell lines
|
|
Species studied:
human
|
|
Enzymes shown to modify site in vitro:
|
|
|
|
Upstream Regulation
|
|
Potential in vivo enzymes for site:
|
|
Type
|
Enzyme
|
Evidence
|
Notes
|
|
KINASE
|
p70S6K (human)
|
pharmacological inhibitor of upstream enzyme
|
|
|
KINASE
|
Akt1 (human)
|
transfection of dominant-negative enzyme, transfection of constitutively active enzyme
|
|
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
rapamycin
|
|
|
|
decrease
|
|
|
PD98059
|
rapamycin
|
|
|
no effect upon treatment-induced decrease
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
molecular association, regulation
|
|
Effect of modification (process):
cell cycle regulation, transcription, altered
|
|
Modification regulates interactions with:
|
|
Interacting molecule
|
Interacting domains
|
Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
|
|
DNA
|
|
Not reported
|
|
|
|
|
|
Comments:
inhibits MAD1-mediated transcription repression
|
