|
Orthologous residues
|
|
TRHR (human): T365‑p, TRHR (mouse): T365‑p, TRHR (rat): T365‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phospho-antibody
|
|
Cellular systems studied:
cell lines, tissue
|
|
Species studied:
hamster, human, rat
|
|
Upstream Regulation
|
|
Potential in vivo enzymes for site:
|
|
Type
|
Enzyme
|
Evidence
|
Notes
|
|
KINASE
|
GRK2 (human)
|
transfection of dominant-negative enzyme, antisense inhibition of upstream enzyme
|
|
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
phorbol ester, ionomycin
|
|
|
|
increase
|
|
|
TRH
|
|
|
|
increase
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
intracellular localization, receptor internalization, altered
|
