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Orthologous residues
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|
PICH (human): T1063‑p, PICH (mouse): T1057‑p
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Characterization
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Methods used to characterize site in vivo:
immunoprecipitation, mass spectrometry, mutation of modification site
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|
Relevant cell lines - cell types - tissues:
HeLa (cervical), U2OS (bone cell) [GR (human)]
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|
Cellular systems studied:
cell lines
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|
Species studied:
human
|
|
Enzymes shown to modify site in vitro:
|
|
|
|
Upstream Regulation
|
|
Potential in vivo enzymes for site:
|
|
Type
|
Enzyme
|
Evidence
|
Notes
|
|
KINASE
|
PLK1 (human)
|
antisense inhibition of upstream enzyme, transfection of constitutively active enzyme, co-immunoprecipitation, transfection of inactive enzyme
|
|
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
siRNA
|
|
|
|
decrease
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
intracellular localization
|
|
Comments:
required for checkpoint signaling and proper sister chromosomes segregation
|
