Curated Information
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Curated Information Page
PubMed Id: 19520772 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Kong X, et al. (2009) CDK11p58 phosphorylation of PAK1 Ser174 promotes DLC2 binding and roles on cell cycle progression. J Biochem 146, 417-27 19520772
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S174-p - PAK1 (human)
Orthologous residues
PAK1 (human): S174‑p, PAK1 (mouse): S174‑p, PAK1 (rat): S174‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  melanoma skin cancer
 Relevant cell lines - cell types - tissues:  A375 (melanocyte), COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK19 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK19 (human) transfection of wild-type enzyme, transfection of dominant-negative enzyme
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, molecular association, regulation
 Effect of modification (process):  cell cycle regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DYNLL2 (human) Induces pull-down assay, co-immunoprecipitation
 Comments:  important for mitotic progression


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