Tong J, et al. (2009) Epidermal growth factor receptor phosphorylation sites Ser991 and Tyr998 are implicated in the regulation of receptor endocytosis and phosphorylations at Ser1039 and Thr1041. Mol Cell Proteomics 8, 2131-44 19531499

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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T693-p - EGFR (human) ▲

Modsite: RELVEPLtPsGEAPN SwissProt Entrez-Gene Orthologous residues EGFR (human): T693‑p, EGFR iso2 (human): ‑, EGFR iso5 (human): T693‑p, EGFR (mouse): T695‑p, EGFR (rat): T694‑p, EGFR (pig): T693‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human

S991-p - EGFR (human) ▲

Modsite: DERMHLPsPtDsNFy SwissProt Entrez-Gene Orthologous residues EGFR (human): S991‑p, EGFR iso2 (human): ‑, EGFR iso5 (human): S991‑p, EGFR (mouse): S993‑p, EGFR (rat): S992‑p, EGFR (pig): S991‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes EGF increase Downstream Regulation Effect of modification (function):  molecular association, regulation, phosphorylation, receptor internalization, altered Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays CBL (human) Induces co-immunoprecipitation ubiquitin (human) Induces co-immunoprecipitation Comments:  mutation of this site increases phosphorylation of EGFR S1039, T1041 and Y1092

Y998-p - EGFR (human) ▲

Modsite: sPtDsNFyRALMDEE SwissProt Entrez-Gene Orthologous residues EGFR (human): Y998‑p, EGFR iso2 (human): ‑, EGFR iso5 (human): Y998‑p, EGFR (mouse): Y1000‑p, EGFR (rat): Y999‑p, EGFR (pig): Y998‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes EGF increase Downstream Regulation Effect of modification (function):  molecular association, regulation, phosphorylation, receptor internalization, altered Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays CBL (human) Induces co-immunoprecipitation Comments:  mutation of this site increases phosphorylation of EGFR S1039, T1041 and Y1092

S1026-p - EGFR (human) ▲

Modsite: PQQGFFssPSTsRtP SwissProt Entrez-Gene Orthologous residues EGFR (human): S1026‑p, EGFR iso2 (human): ‑, EGFR iso5 (human): S1026‑p, EGFR (mouse): S1028‑p, EGFR (rat): S1027‑p, EGFR (pig): S1026‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human

S1037-p - EGFR (human) ▲

Modsite: sRtPLLSsLsAtsNN SwissProt Entrez-Gene Orthologous residues EGFR (human): S1037‑p, EGFR iso2 (human): ‑, EGFR iso5 (human): S1037‑p, EGFR (mouse): S1039‑p, EGFR (rat): S1038‑p, EGFR (pig): S1037‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human

S1039-p - EGFR (human) ▲

Modsite: tPLLSsLsAtsNNst SwissProt Entrez-Gene Orthologous residues EGFR (human): S1039‑p, EGFR iso2 (human): ‑, EGFR iso5 (human): S1039‑p, EGFR (mouse): S1041‑p, EGFR (rat): S1040‑p, EGFR (pig): S1039‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE P38A (human) pharmacological inhibitor of upstream enzyme KINASE P38B (human) pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes EGFR (human) increase EGFR Y998F/S991A mutant EGF increase SB202190 EGF inhibit treatment-induced increase

T1041-p - EGFR (human) ▲

Modsite: LLSsLsAtsNNstVA SwissProt Entrez-Gene Orthologous residues EGFR (human): T1041‑p, EGFR iso2 (human): ‑, EGFR iso5 (human): T1041‑p, EGFR (mouse): T1043‑p, EGFR (rat): N1042‑p, EGFR (pig): T1041‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE P38A (human) pharmacological inhibitor of upstream enzyme KINASE P38B (human) pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes EGFR (human) increase EGFR Y998F/S991A mutant EGF increase SB202190 EGF inhibit treatment-induced increase

S1042-p - EGFR (human) ▲
Modsite: LSsLsAtsNNstVAC SwissProt Entrez-Gene Orthologous residues EGFR (human): S1042‑p, EGFR iso2 (human): ‑, EGFR iso5 (human): S1042‑p, EGFR (mouse): S1044‑p, EGFR (rat): S1043‑p, EGFR (pig): S1042‑p

Y1069-p - EGFR (human) ▲

Modsite: EDsFLQRyssDPtGA SwissProt Entrez-Gene Orthologous residues EGFR (human): Y1069‑p, EGFR iso2 (human): ‑, EGFR iso5 (human): Y1069‑p, EGFR (mouse): Y1069‑p, EGFR (rat): Y1068‑p, EGFR (pig): Y1068‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human

S1081-p - EGFR (human) ▲

Modsite: tGALtEDsIDDtFLP SwissProt Entrez-Gene Orthologous residues EGFR (human): S1081‑p, EGFR iso2 (human): ‑, EGFR iso5 (human): S1081‑p, EGFR (mouse): N1081‑p, EGFR (rat): N1080‑p, EGFR (pig): S1080‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human

T1085-p - EGFR (human) ▲

Modsite: tEDsIDDtFLPVPEy SwissProt Entrez-Gene Orthologous residues EGFR (human): T1085‑p, EGFR iso2 (human): ‑, EGFR iso5 (human): T1085‑p, EGFR (mouse): A1085‑p, EGFR (rat): T1084‑p, EGFR (pig): T1084‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human

Y1092-p - EGFR (human) ▲

Modsite: tFLPVPEyINQsVPk SwissProt Entrez-Gene Orthologous residues EGFR (human): Y1092‑p, EGFR iso2 (human): ‑, EGFR iso5 (human): ‑, EGFR (mouse): Y1092‑p, EGFR (rat): Y1091‑p, EGFR (pig): Y1091‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes EGF increase

S1096-p - EGFR (human) ▲

Modsite: VPEyINQsVPkRPAG SwissProt Entrez-Gene Orthologous residues EGFR (human): S1096‑p, EGFR iso2 (human): ‑, EGFR iso5 (human): ‑, EGFR (mouse): S1096‑p, EGFR (rat): S1095‑p, EGFR (pig): S1095‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human

S1104-p - EGFR (human) ▲

Modsite: VPkRPAGsVQNPVyH SwissProt Entrez-Gene Orthologous residues EGFR (human): S1104‑p, EGFR iso2 (human): ‑, EGFR iso5 (human): ‑, EGFR (mouse): S1104‑p, EGFR (rat): S1103‑p, EGFR (pig): S1103‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human

Y1110-p - EGFR (human) ▲

Modsite: GsVQNPVyHNQPLNP SwissProt Entrez-Gene Orthologous residues EGFR (human): Y1110‑p, EGFR iso2 (human): ‑, EGFR iso5 (human): ‑, EGFR (mouse): Y1110‑p, EGFR (rat): Y1109‑p, EGFR (pig): Y1109‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human

Y1138-p - EGFR (human) ▲

Modsite: tAVGNPEyLNtVQPt SwissProt Entrez-Gene Orthologous residues EGFR (human): Y1138‑p, EGFR iso2 (human): ‑, EGFR iso5 (human): ‑, EGFR (mouse): Y1138‑p, EGFR (rat): Y1137‑p, EGFR (pig): Y1137‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human

S1166-p - EGFR (human) ▲

Modsite: QkGsHQIsLDNPDyQ SwissProt Entrez-Gene Orthologous residues EGFR (human): S1166‑p, EGFR iso2 (human): ‑, EGFR iso5 (human): ‑, EGFR (mouse): S1166‑p, EGFR (rat): S1165‑p, EGFR (pig): N1165‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human

Y1172-p - EGFR (human) ▲

Modsite: IsLDNPDyQQDFFPk SwissProt Entrez-Gene Orthologous residues EGFR (human): Y1172‑p, EGFR iso2 (human): ‑, EGFR iso5 (human): ‑, EGFR (mouse): Y1172‑p, EGFR (rat): Y1171‑p, EGFR (pig): Y1171‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human

Y1197-p - EGFR (human) ▲

Modsite: stAENAEyLRVAPQS SwissProt Entrez-Gene Orthologous residues EGFR (human): Y1197‑p, EGFR iso2 (human): ‑, EGFR iso5 (human): ‑, EGFR (mouse): Y1197‑p, EGFR (rat): Y1196‑p, EGFR (pig): Y1196‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human