Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Curated Information Page
PubMed Id: 12149249 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Conus NM, et al. (2002) Direct identification of tyrosine 474 as a regulatory phosphorylation site for the Akt protein kinase. J Biol Chem 277, 38021-8 12149249
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

S124-p - Akt1 (human)
Orthologous residues
Akt1 (human): S124‑p, Akt1 (mouse): S124‑p, Akt1 (rat): S124‑p, Akt1 (fruit fly): T236‑p, Akt1 (cow): S124‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, phospho-antibody, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), SKOV-3 (ovarian)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey

S129-p - Akt1 (human)
Orthologous residues
Akt1 (human): S129‑p, Akt1 (mouse): S129‑p, Akt1 (rat): S129‑p, Akt1 (fruit fly): E241‑p, Akt1 (cow): S129‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, phospho-antibody, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), SKOV-3 (ovarian)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey

T308-p - Akt1 (human)
Orthologous residues
Akt1 (human): T308‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), SKOV-3 (ovarian)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
vanadate increase
IGF-1 increase

T450-p - Akt1 (human)
Orthologous residues
Akt1 (human): T450‑p, Akt1 (mouse): T450‑p, Akt1 (rat): T450‑p, Akt1 (fruit fly): T565‑p, Akt1 (cow): T450‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, phospho-antibody, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), SKOV-3 (ovarian)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey

S473-p - Akt1 (human)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), SKOV-3 (ovarian)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
vanadate increase
IGF-1 increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

Y474-p - Akt1 (human)
Orthologous residues
Akt1 (human): Y474‑p, Akt1 (mouse): Y474‑p, Akt1 (rat): Y474‑p, Akt1 (fruit fly): Y587‑p, Akt1 (cow): Y474‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), SKOV-3 (ovarian)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
vanadate increase
IGF-1 increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced


Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.