Curated Information
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Curated Information Page
PubMed Id: 16908529 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Yajima H, Lee KJ, Chen BP (2006) ATR-dependent phosphorylation of DNA-dependent protein kinase catalytic subunit in response to UV-induced replication stress. Mol Cell Biol 26, 7520-8 16908529
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S2056-p - DNA-PK (human) ▲
Orthologous residues
DNA‑PK (human): S2056‑p, DNA‑PK (mouse): S2053‑p, DNA‑PK (rat): S2051‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  CHO (fibroblast), HeLa (cervical), HSF (fibroblast), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  hamster, human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
UV no change compared to control

T2609-p - DNA-PK (human) ▲
Orthologous residues
DNA‑PK (human): T2609‑p, DNA‑PK (mouse): T2605‑p, DNA‑PK (rat): T2603‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  CHO (fibroblast), HeLa (cervical), HSF (fibroblast), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  hamster, human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATR (human) co-immunoprecipitation, pharmacological activator of upstream enzyme, genetic transfer of dominant-negative enzyme, siRNA inhibition of enzyme, phospho-antibody, pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
UV increase
wortmannin UV no effect upon treatment-induced increase
caffeine UV inhibit treatment-induced increase
siRNA UV no effect upon treatment-induced increase ATM siRNA
siRNA UV inhibit treatment-induced increase ATR siRNA

T2647-p - DNA-PK (human) ▲
Orthologous residues
DNA‑PK (human): T2647‑p, DNA‑PK (mouse): T2643‑p, DNA‑PK (rat): T2641‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  CHO (fibroblast), HeLa (cervical), HSF (fibroblast), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  hamster, human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATR (human) co-immunoprecipitation, pharmacological activator of upstream enzyme, genetic transfer of dominant-negative enzyme, siRNA inhibition of enzyme, phospho-antibody, pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
UV increase
wortmannin UV no effect upon treatment-induced increase
caffeine UV inhibit treatment-induced increase
siRNA UV no effect upon treatment-induced increase ATM siRNA
siRNA UV inhibit treatment-induced increase ATR siRNA


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