Curated Information
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Curated Information Page
PubMed Id: 17018282 
Yang L, Lin C, Liu ZR (2006) P68 RNA helicase mediates PDGF-induced epithelial mesenchymal transition by displacing Axin from beta-catenin. Cell 127, 139-55 17018282
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S33-p - CTNNB1 (human)
Orthologous residues
CTNNB1 (human): S33‑p, CTNNB1 (mouse): S33‑p, CTNNB1 (rat): S33‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  HT-29 (intestinal)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DDX5 (human) decrease DDX5 phosphorylated on Y593 inhibits CTNNB phosphorylation, thereby promoting its nuclear translocation,
Downstream Regulation
 Effect of modification (function):  intracellular localization

S37-p - CTNNB1 (human)
Orthologous residues
CTNNB1 (human): S37‑p, CTNNB1 (mouse): S37‑p, CTNNB1 (rat): S37‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  HT-29 (intestinal)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DDX5 (human) decrease DDX5 phosphorylated on Y593 inhibits CTNNB phosphorylation, thereby promoting its nuclear translocation,
Downstream Regulation
 Effect of modification (function):  intracellular localization

T41-p - CTNNB1 (human)
Orthologous residues
CTNNB1 (human): T41‑p, CTNNB1 (mouse): T41‑p, CTNNB1 (rat): T41‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Relevant cell lines - cell types - tissues:  HT-29 (intestinal)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DDX5 (human) decrease DDX5 phosphorylated on Y593 inhibits CTNNB phosphorylation, thereby promoting its nuclear translocation,
Downstream Regulation
 Effect of modification (function):  intracellular localization

Y593-p - DDX5 (human)
Orthologous residues
DDX5 (human): Y593‑p, DDX5 (mouse): Y593‑p, DDX5 (rat): Y593‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HT-29 (intestinal), MDCK (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Abl (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Abl (human) co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PDGF increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cytoskeletal reorganization
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CTNNB1 (human) Induces intracellular localization, phosphorylation co-immunoprecipitation
 Comments:  promotes PDGF-stimulated epithelial-mesenchymal transition; inhibits CTNNB phosphorylation on S33, S37, and T41, thereby promoting its translocation to the nucleus.


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