Curated Information
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Curated Information Page
PubMed Id: 17046689 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Zhou Z, et al. (2006) Brain-specific phosphorylation of MeCP2 regulates activity-dependent Bdnf transcription, dendritic growth, and spine maturation. Neuron 52, 255-69 17046689
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

S80-p - MeCP2 (rat) ▲
Orthologous residues
MeCP2 (human): S80‑p, MeCP2 (mouse): S80‑p, MeCP2 iso2 (mouse): S97‑p, MeCP2 (rat): S80‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cortical', 293T (epithelial), brain
 Cellular systems studied:  cell lines, tissue
 Species studied:  human, mouse, rat

S229-p - MeCP2 (rat) ▲
Orthologous residues
MeCP2 (human): S229‑p, MeCP2 (mouse): S229‑p, MeCP2 iso2 (mouse): S246‑p, MeCP2 (rat): S229‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cortical', 293T (epithelial), brain
 Cellular systems studied:  cell lines, tissue
 Species studied:  human, mouse, rat

S421-p - MeCP2 (rat) ▲
Orthologous residues
MeCP2 (human): S423‑p, MeCP2 (mouse): S421‑p, MeCP2 iso2 (mouse): S438‑p, MeCP2 (rat): S421‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, cortical', 293T (epithelial), brain
 Cellular systems studied:  cell lines, tissue
 Species studied:  human, mouse, rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CAMK2A (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CAMK2A (mouse) phospho-antibody, pharmacological activator of upstream enzyme, transfection of dominant-negative enzyme, pharmacological inhibitor of upstream enzyme, transfection of constitutively active enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
KCl increase
KN-93 KCl inhibit treatment-induced increase
KN-92 KCl no effect upon treatment-induced increase
glutamate increase
nimodipine glutamate inhibit treatment-induced increase
APV glutamate inhibit treatment-induced increase
CNQX glutamate inhibit treatment-induced increase
EGTA glutamate inhibit treatment-induced increase
NMDA increase
APV NMDA inhibit treatment-induced increase
EGTA NMDA inhibit treatment-induced increase
nimodipine NMDA inhibit treatment-induced increase
KN-93 NMDA inhibit treatment-induced increase
KN-92 NMDA no effect upon treatment-induced increase
bicuculline increase
nimodipine bicuculline inhibit treatment-induced increase
APV bicuculline inhibit treatment-induced increase
CNQX bicuculline inhibit treatment-induced increase
BDNF increase
EGF no change compared to control
IGF-1 no change compared to control
NGF no change compared to control
increase NT3
increase NT4
PDGF no change compared to control
no change compared to control CNTF
increase Metrazole (pentylenetetrazole)
kainic acid increase
light increase
Downstream Regulation
 Effect of modification (function):  activation
 Effect of modification (process):  cell differentiation, altered, transcription, altered


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