Curated Information
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Curated Information Page
PubMed Id: 16964247 
Yang WH, et al. (2006) Modification of p53 with O-linked N-acetylglucosamine regulates p53 activity and stability. Nat Cell Biol 8, 1074-83 16964247
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S149-gl - p53 (human)
Orthologous residues
p53 (human): S149‑gl, p53 (mouse): A143‑gl, p53 iso2 (mouse): A146‑gl, p53 (rat): S147‑gl, p53 (rabbit): S146‑gl, p53 (monkey): S149‑gl
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
 Disease tissue studied:  breast cancer, lung cancer, non-small cell lung cancer
 Relevant cell lines - cell types - tissues:  MCF-7 (breast cell), NCI-H1299 (pulmonary)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
streptozotocin increase
Downstream Regulation
 Effect of modification (function):  phosphorylation, protein stabilization, ubiquitination
 Comments:  decreases p53 T155 phosphorylation; decreases p53 ubiquitination

T155-p - p53 (human)
Orthologous residues
p53 (human): T155‑p, p53 (mouse): S149‑p, p53 iso2 (mouse): S152‑p, p53 (rat): T153‑p, p53 (rabbit): T152‑p, p53 (monkey): S155‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MCF-7 (breast cell)
 Cellular systems studied:  cell lines
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
doxorubicin increase
PUGNAc doxorubicin inhibit treatment-induced increase
streptozotocin doxorubicin no effect upon treatment-induced increase
PUGNAc decrease
streptozotocin decrease not observed in p53 S149A mutant
Downstream Regulation
 Effect of modification (function):  protein degradation
 Comments:  reduced by O-GlcNAc glycosylation of p53 S149

S149-p - p53 (mouse)
Orthologous residues
p53 (human): T155‑p, p53 (mouse): S149‑p, p53 iso2 (mouse): S152‑p, p53 (rat): T153‑p, p53 (rabbit): T152‑p, p53 (monkey): S155‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody
 Disease tissue studied:  kidney cancer
 Relevant cell lines - cell types - tissues:  heart, kidney, liver, pancreas
 Cellular systems studied:  tissue
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
streptozotocin decrease


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