Curated Information
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Curated Information Page
PubMed Id: 10839545 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Wu X, et al. (2000) ATM phosphorylation of Nijmegen breakage syndrome protein is required in a DNA damage response. Nature 405, 477-82 10839545
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S343-p - NBS1 (human)
Orthologous residues
NBS1 (human): S343‑p, NBS1 (mouse): S343‑p, NBS1 (rat): S343‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], BJAB (B lymphocyte), L6 (myoblast)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ATM (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) genetic knockout/knockin of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
Downstream Regulation
 Comments:  S343A mutant failed to protect cells from gamma-irradiation-induced death

S397-p - NBS1 (human)
Orthologous residues
NBS1 (human): S397‑p, NBS1 (mouse): S398‑p, NBS1 (rat): L398‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], BJAB (B lymphocyte), L6 (myoblast)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ATM (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) genetic knockout/knockin of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
Downstream Regulation
 Comments:  S397A mutant failed to protect cells from gamma-irradiation-induced death

S432-p - NBS1 (human)
Orthologous residues
NBS1 (human): S432‑p, NBS1 (mouse): S433‑p, NBS1 (rat): S433‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  BJAB (B lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human

S509-p - NBS1 (human)
Orthologous residues
NBS1 (human): S509‑p, NBS1 (mouse): S508‑p, NBS1 (rat): S508‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  BJAB (B lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human

S604-p - NBS1 (human)
Orthologous residues
NBS1 (human): S604‑p, NBS1 (mouse): N602‑p, NBS1 (rat): N602‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  BJAB (B lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human

S611-p - NBS1 (human)
Orthologous residues
NBS1 (human): S611‑p, NBS1 (mouse): S609‑p, NBS1 (rat): S609‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  BJAB (B lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human

S612-p - NBS1 (human)
Orthologous residues
NBS1 (human): S612‑p, NBS1 (mouse): N610‑p, NBS1 (rat): N610‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  BJAB (B lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human

S615-p - NBS1 (human)
Orthologous residues
NBS1 (human): S615‑p, NBS1 (mouse): L613‑p, NBS1 (rat): L613‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], BJAB (B lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ATM (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) genetic knockout/knockin of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
Downstream Regulation
 Comments:  S615A mutant failed to protect cells from gamma-irradiation-induced death


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