Curated Information
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PubMed Id: 10811803 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Zhang W, et al. (2000) Association of Grb2, Gads, and phospholipase C-gamma 1 with phosphorylated LAT tyrosine residues. Effect of LAT tyrosine mutations on T cell angigen receptor-mediated signaling. J Biol Chem 275, 23355-61 10811803
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y161-p - LAT (human)
Orthologous residues
LAT (human): Y161‑p, LAT iso2 (human): Y132‑p, LAT (mouse): Y136‑p, LAT (rat): Y136‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte) [LAT (human)]
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PLCG1 (human) Induces molecular association, regulation co-immunoprecipitation

Y200-p - LAT (human)
Orthologous residues
LAT (human): Y200‑p, LAT iso2 (human): Y171‑p, LAT (mouse): Y175‑p, LAT (rat): Y175‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte) [LAT (human)]
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 increase
Downstream Regulation
 Effect of modification (function):  activity, induced, molecular association, regulation
 Effect of modification (process):  transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
GADS (human) Induces molecular association, regulation co-immunoprecipitation
Grb2 (human) Induces molecular association, regulation chemical cross-linking
PLCG1 (human) Induces molecular association, regulation co-immunoprecipitation
 Comments:  NF-AT mediated transcription

Y220-p - LAT (human)
Orthologous residues
LAT (human): Y220‑p, LAT iso2 (human): Y191‑p, LAT (mouse): Y195‑p, LAT (rat): Y195‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte) [LAT (human)]
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 increase
Downstream Regulation
 Effect of modification (function):  activity, induced, molecular association, regulation
 Effect of modification (process):  transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
GADS (human) Induces molecular association, regulation co-immunoprecipitation
Grb2 (human) Induces molecular association, regulation chemical cross-linking
PLCG1 (human) Induces molecular association, regulation co-immunoprecipitation
 Comments:  NF-AT mediated transcription

Y255-p - LAT (human)
Orthologous residues
LAT (human): Y255‑p, LAT iso2 (human): Y226‑p, LAT (mouse): Y235‑p, LAT (rat): Y234‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte) [LAT (human)]
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 increase
Downstream Regulation
 Effect of modification (function):  activity, induced, molecular association, regulation
 Effect of modification (process):  transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Grb2 (human) Induces molecular association, regulation chemical cross-linking
PLCG1 (human) Induces molecular association, regulation co-immunoprecipitation
 Comments:  NF-AT mediated transcription


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