Curated Information

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Curated Information Page

PubMed Id: 15054037

This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus^®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus^®, click here.

Information from this record has been curated, but not yet edited in PhosphoSitePlus^® and may be incomplete.

Mangin P, et al. (2004) Identification of a novel 14-3-3zeta binding site within the cytoplasmic tail of platelet glycoprotein Ibalpha. Blood 104, 420-7 15054037

Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.

S603-p - GPIbA (human)

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Orthologous residues

GPIbA (human): S603‑p, GPIbA (mouse): S711‑p

Characterization

Methods used to characterize site in vivo: mutation of modification site, phosphoamino acid analysis, western blotting

Relevant cell lines - cell types - tissues: CHO (fibroblast), platelet-blood

Cellular systems studied: cell lines, primary cells

Species studied: hamster, human

Upstream Regulation

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
von Willebrand factor				decrease

S606-p - GPIbA (human)

▲

Orthologous residues

GPIbA (human): S606‑p, GPIbA (mouse): S714‑p

Characterization

Methods used to characterize site in vivo: mutation of modification site, phosphoamino acid analysis, western blotting

Relevant cell lines - cell types - tissues: CHO (fibroblast), platelet-blood

Cellular systems studied: cell lines, primary cells

Species studied: hamster, human

Upstream Regulation

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
von Willebrand factor				decrease

Downstream Regulation

Effect of modification (function): molecular association, regulation

Effect of modification (process): cytoskeletal reorganization

Modification regulates interactions with:

Interacting molecule	Interacting domains	Effect	Consequences (function)	Consequences (process)	Detection assays
14-3-3 zeta (human)		Induces			sequence-specific competitor, co-immunoprecipitation

Comments: cell spreading (not adhering efficiency)

S625-p - GPIbA (human)

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Orthologous residues

GPIbA (human): S625‑p, GPIbA (mouse): S733‑p

Characterization

Methods used to characterize site in vivo: mutation of modification site, phosphoamino acid analysis, western blotting

Relevant cell lines - cell types - tissues: CHO (fibroblast), platelet-blood

Cellular systems studied: cell lines, primary cells

Species studied: hamster, human

Upstream Regulation

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
von Willebrand factor				decrease

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