Curated Information
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PubMed Id: 9430688 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Dalby KN, et al. (1998) Identification of regulatory phosphorylation sites in mitogen-activated protein kinase (MAPK)-activated protein kinase-1a/p90rsk that are inducible by MAPK. J Biol Chem 273, 1496-505 9430688
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S221-p - p90RSK (rat) ▲
Orthologous residues
p90RSK (human): S221‑p, p90RSK iso3 (human): S230‑p, p90RSK (mouse): S221‑p, p90RSK iso3 (mouse): S227‑p, p90RSK (rat): S221‑p, p90RSK (chicken): S239‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE p90RSK (human) transfection of wild-type enzyme, transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
PD98059 decrease

T359-p - p90RSK (rat) ▲
Orthologous residues
p90RSK (human): T359‑p, p90RSK iso3 (human): T368‑p, p90RSK (mouse): T348‑p, p90RSK iso3 (mouse): ‑, p90RSK (rat): T359‑p, p90RSK (chicken): T377‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ERK2 (human)
KINASE ERK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
PD98059 decrease

S363-p - p90RSK (rat) ▲
Orthologous residues
p90RSK (human): S363‑p, p90RSK iso3 (human): S372‑p, p90RSK (mouse): S352‑p, p90RSK iso3 (mouse): ‑, p90RSK (rat): S363‑p, p90RSK (chicken): S381‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ERK2 (human)
KINASE ERK1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
PD98059 decrease
Downstream Regulation
 Effect of modification (function):  activation

S380-p - p90RSK (rat) ▲
Orthologous residues
p90RSK (human): S380‑p, p90RSK iso3 (human): S389‑p, p90RSK (mouse): S369‑p, p90RSK iso3 (mouse): ‑, p90RSK (rat): S380‑p, p90RSK (chicken): S398‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE p90RSK (human) transfection of wild-type enzyme, transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
PD98059 decrease
Downstream Regulation
 Effect of modification (function):  activation

T573-p - p90RSK (rat) ▲
Orthologous residues
p90RSK (human): T573‑p, p90RSK iso3 (human): T582‑p, p90RSK (mouse): T562‑p, p90RSK iso3 (mouse): ‑, p90RSK (rat): T573‑p, p90RSK (chicken): T590‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ERK1 (human)
KINASE ERK2 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
PD98059 decrease
Downstream Regulation
 Effect of modification (function):  activation

S732-p - p90RSK (rat) ▲
Orthologous residues
p90RSK (human): S732‑p, p90RSK iso3 (human): S741‑p, p90RSK (mouse): S721‑p, p90RSK iso3 (mouse): ‑, p90RSK (rat): S732‑p, p90RSK (chicken): S749‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE p90RSK (human) transfection of wild-type enzyme, transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol ester increase
PD98059 decrease


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