Curated Information
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Curated Information Page
PubMed Id: 18930133 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Sun W, et al. (2009) PPM1A and PPM1B act as IKKbeta phosphatases to terminate TNFalpha-induced IKKbeta-NF-kappaB activation. Cell Signal 21, 95-102 18930133
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S177-p - IKKB (human)
Orthologous residues
IKKB (human): S177‑p, IKKB (mouse): S177‑p, IKKB (rat): S177‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPPM1A (human) transfection of wild-type enzyme, siRNA inhibition of enzyme, phospho-antibody, co-immunoprecipitation, transfection of inactive enzyme
PHOSPHATASE PPM1B (human) transfection of wild-type enzyme, siRNA inhibition of enzyme, phospho-antibody, co-immunoprecipitation, transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Effect of modification (process):  transcription, induced

S181-p - IKKB (human)
Orthologous residues
IKKB (human): S181‑p, IKKB (mouse): S181‑p, IKKB (rat): S181‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPM1B (human) transfection of wild-type enzyme, siRNA inhibition of enzyme, phospho-antibody, co-immunoprecipitation, transfection of inactive enzyme
PHOSPHATASE PPPM1A (human) transfection of wild-type enzyme, siRNA inhibition of enzyme, phospho-antibody, co-immunoprecipitation, transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Effect of modification (process):  transcription, induced


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