Curated Information
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Curated Information Page
PubMed Id: 10779345 
Mothe-Satney I, et al. (2000) Multiple mechanisms control phosphorylation of PHAS-I in five (S/T)P sites that govern translational repression. Mol Cell Biol 20, 3558-67 10779345
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T36-p - 4E-BP1 (rat)
Orthologous residues
4E‑BP1 (human): T37‑p, 4E‑BP1 (mouse): T36‑p, 4E‑BP1 (rat): T36‑p, 4E‑BP1 (fruit fly): T37‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
amino acids increase
insulin amino acids augment treatment-induced increase
rapamycin amino acids inhibit treatment-induced increase
rapamycin no change compared to control
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, phosphorylation
 Effect of modification (process):  translation, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
eIF4E (human) Disrupts far-Western, pull-down assay
 Comments:  is required for phosphorylation of S63

T45-p - 4E-BP1 (rat)
Orthologous residues
4E‑BP1 (human): T46‑p, 4E‑BP1 (mouse): T45‑p, 4E‑BP1 (rat): T45‑p, 4E‑BP1 (fruit fly): T46‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
amino acids increase
insulin amino acids augment treatment-induced increase
rapamycin amino acids inhibit treatment-induced increase
rapamycin no change compared to control
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, phosphorylation
 Effect of modification (process):  translation, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
eIF4E (human) Disrupts far-Western, pull-down assay
 Comments:  is required for phosphorylation of S63

S64-p - 4E-BP1 (rat)
Orthologous residues
4E‑BP1 (human): S65‑p, 4E‑BP1 (mouse): S64‑p, 4E‑BP1 (rat): S64‑p, 4E‑BP1 (fruit fly): S65‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
amino acids increase
insulin amino acids augment treatment-induced increase
rapamycin insulin inhibit treatment-induced increase
rapamycin no change compared to control
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  translation, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
eIF4E (human) Disrupts far-Western, pull-down assay

T69-p - 4E-BP1 (rat)
Orthologous residues
4E‑BP1 (human): T70‑p, 4E‑BP1 (mouse): T69‑p, 4E‑BP1 (rat): T69‑p, 4E‑BP1 (fruit fly): T70‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
amino acids increase
insulin amino acids augment treatment-induced increase
rapamycin amino acids inhibit treatment-induced increase
rapamycin decrease
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, phosphorylation
 Effect of modification (process):  translation, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
eIF4E (human) Disrupts far-Western, pull-down assay
 Comments:  is required for phosphorylation of S63

S82-p - 4E-BP1 (rat)
Orthologous residues
4E‑BP1 (human): S83‑p, 4E‑BP1 (mouse): S82‑p, 4E‑BP1 (rat): S82‑p, 4E‑BP1 (fruit fly): T83‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
amino acids no change compared to control
insulin no change compared to control
rapamycin no change compared to control
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  translation, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
eIF4E (human) Disrupts far-Western, pull-down assay


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