Curated Information
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Curated Information Page
PubMed Id: 15311280 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Viard P, et al. (2004) PI3K promotes voltage-dependent calcium channel trafficking to the plasma membrane. Nat Neurosci 7, 939-46 15311280
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T308-p - Akt1 (human)
Orthologous residues
Akt1 (human): T308‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, dorsal root ganglion'-brain, COS (fibroblast)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  monkey, rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PIK3CG (human) increase

S473-p - Akt1 (human)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'neuron, dorsal root ganglion'-brain, COS (fibroblast)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  monkey, rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase
PIK3CG (human) increase

S625-p - CACNB2 (rat)
Orthologous residues
CACNB2 (human): S630‑p, CACNB2 iso2 (human): S575‑p, CACNB2 iso3 (human): S576‑p, CACNB2 iso6 (human): S537‑p, CACNB2 iso8 (human): S606‑p, CACNB2 (mouse): S625‑p, CACNB2 (rat): S625‑p, CACNB2 iso2 (rat): S574‑p, CACNB2 (rabbit): S602‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) co-immunoprecipitation, activation of upstream enzyme, transfection of constitutively active enzyme, modification site within consensus motif, transfection of dominant-negative enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PIK3CG (human) increase
Downstream Regulation
 Effect of modification (function):  intracellular localization


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