Curated Information
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Curated Information Page
PubMed Id: 10473617 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Matthews SA, Rozengurt E, Cantrell D (1999) Characterization of serine 916 as an in vivo autophosphorylation site for protein kinase D/Protein kinase Cmu. J Biol Chem 274, 26543-9 10473617
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S910-p - PKD1 (human) ▲
Orthologous residues
PKD1 (human): S910‑p, PKD1 (mouse): S916‑p, PKD1 (rat): S916‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  T lymphocyte-blood
 Cellular systems studied:  primary cells
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 increase
phorbol ester increase

S744-p - PKD1 (mouse) ▲
Orthologous residues
PKD1 (human): S738‑p, PKD1 (mouse): S744‑p, PKD1 (rat): S744‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

S748-p - PKD1 (mouse) ▲
Orthologous residues
PKD1 (human): S742‑p, PKD1 (mouse): S748‑p, PKD1 (rat): S748‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

S916-p - PKD1 (mouse) ▲
Orthologous residues
PKD1 (human): S910‑p, PKD1 (mouse): S916‑p, PKD1 (rat): S916‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  A20 (B lymphocyte), COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey, mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE PKD1 (mouse)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKD1 (mouse) transfection of inactive enzyme, pharmacological activator of upstream enzyme, transfection of constitutively active enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgG increase
phorbol ester increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced


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