Curated Information
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Curated Information Page
PubMed Id: 9697839 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Fu C, Turck CW, Kurosaki T, Chan AC (1998) BLNK: a central linker protein in B cell activation. Immunity 9, 93-103 9697839
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y72-p - BLNK (human) ▲
Orthologous residues
BLNK (human): Y72‑p, BLNK iso3 (human): ‑, BLNK (mouse): Y72‑p, BLNK (rat): Y72‑p, BLNK (chicken): Y91‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  A20 (B lymphocyte), Daudi (B lymphocyte), SF9
 Cellular systems studied:  cell lines
 Species studied:  human, insect, mouse
 Comments:  A BLNK mutant in which Y72, Y84, Y96, and Y178 were converted to F was used in this study.
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Syk (human) transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgM increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PLCG2 (mouse) Induces phosphorylation co-immunoprecipitation
PLCG1 (mouse) Induces phosphorylation co-immunoprecipitation

Y84-p - BLNK (human) ▲
Orthologous residues
BLNK (human): Y84‑p, BLNK iso3 (human): ‑, BLNK (mouse): Y84‑p, BLNK (rat): Y84‑p, BLNK (chicken): Y103‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  A20 (B lymphocyte), Daudi (B lymphocyte), SF9
 Cellular systems studied:  cell lines
 Species studied:  human, insect, mouse
 Comments:  A BLNK mutant in which Y72, Y84, Y96, and Y178 were converted to F was used in this study.
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Syk (human) transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgM increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PLCG1 (mouse) Induces phosphorylation co-immunoprecipitation
PLCG2 (mouse) Induces phosphorylation co-immunoprecipitation

Y96-p - BLNK (human) ▲
Orthologous residues
BLNK (human): Y96‑p, BLNK iso3 (human): ‑, BLNK (mouse): Y96‑p, BLNK (rat): Y96‑p, BLNK (chicken): Y115‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  A20 (B lymphocyte), Daudi (B lymphocyte), SF9
 Cellular systems studied:  cell lines
 Species studied:  human, insect, mouse
 Comments:  A BLNK mutant in which Y72, Y84, Y96, and Y178 were converted to F was used in this study.
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Syk (human) transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgM increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PLCG1 (mouse) Induces phosphorylation co-immunoprecipitation
PLCG2 (mouse) Induces phosphorylation co-immunoprecipitation

Y178-p - BLNK (human) ▲
Orthologous residues
BLNK (human): Y178‑p, BLNK iso3 (human): ‑, BLNK (mouse): Y178‑p, BLNK (rat): Y178‑p, BLNK (chicken): Y194‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  A20 (B lymphocyte), Daudi (B lymphocyte), SF9
 Cellular systems studied:  cell lines
 Species studied:  human, insect, mouse
 Comments:  A BLNK mutant in which Y72, Y84, Y96, and Y178 were converted to F was used in this study.
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Syk (human) transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-IgM increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PLCG1 (mouse) Induces phosphorylation co-immunoprecipitation
PLCG2 (mouse) Induces phosphorylation co-immunoprecipitation


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