|
Orthologous residues
|
|
SHP‑2 (human): Y546‑p, SHP‑2 (mouse): Y546‑p, SHP‑2 (rat): Y546‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphoamino acid analysis, phosphopeptide mapping, western blotting
|
|
Relevant cell lines - cell types - tissues:
3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], AT1009, ATWT
|
|
Cellular systems studied:
cell lines
|
|
Species studied:
dog
|
|
Enzymes shown to modify site in vitro:
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
molecular association, regulation
|
|
Modification regulates interactions with:
|
|
Interacting molecule
|
Interacting domains
|
Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
|
|
Grb2 (mouse)
|
|
Induces
|
molecular association, regulation
|
|
co-immunoprecipitation, electrophoretic visualization
|
|
|
Comments:
Following PDGF stimulation, Grb2 binds tyrosine-phosphorylated SHPTP2
|
