Curated Information
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PubMed Id: 11466320 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Kalo MS, Yu HH, Pasquale EB (2001) In vivo tyrosine phosphorylation sites of activated ephrin-B1 and ephB2 from neural tissue. J Biol Chem 276, 38940-8 11466320
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y312-p - EFNB1 (chicken)
Orthologous residues
EFNB1 (human): Y324‑p, EFNB1 (mouse): Y323‑p, EFNB1 (rat): Y323‑p, EFNB1 (chicken): Y312‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  'retina, embryo', 293T (epithelial)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  chicken, human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EphB2 increase

Y317-p - EFNB1 (chicken)
Orthologous residues
EFNB1 (human): Y329‑p, EFNB1 (mouse): Y328‑p, EFNB1 (rat): Y328‑p, EFNB1 (chicken): Y317‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  'retina, embryo', 293T (epithelial)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  chicken, human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EphB2 increase

Y331-p - EFNB1 (chicken)
Orthologous residues
EFNB1 (human): Y343‑p, EFNB1 (mouse): Y342‑p, EFNB1 (rat): Y342‑p, EFNB1 (chicken): Y331‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  'retina, embryo', 293T (epithelial)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  chicken, human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EphB2 increase

Y579-p - EphB2 (chicken)
Orthologous residues
EphB2 (human): Y577‑p, EphB2 iso2 (human): Y577‑p, EphB2 (mouse): Y585‑p, EphB2 (rat): Y690‑p, EphB2 (chicken): Y579‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody
 Relevant cell lines - cell types - tissues:  'retina, embryo', 293T (epithelial)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  chicken, human

Y586-p - EphB2 (chicken)
Orthologous residues
EphB2 (human): Y584‑p, EphB2 iso2 (human): Y584‑p, EphB2 (mouse): Y592‑p, EphB2 (rat): Y697‑p, EphB2 (chicken): Y586‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody
 Relevant cell lines - cell types - tissues:  'retina, embryo'
 Cellular systems studied:  primary cells
 Species studied:  chicken

Y614-p - EphB2 (chicken)
Orthologous residues
EphB2 (human): Y596‑p, EphB2 iso2 (human): Y596‑p, EphB2 (mouse): Y604‑p, EphB2 (rat): Y709‑p, EphB2 (chicken): Y614‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody
 Relevant cell lines - cell types - tissues:  'retina, embryo', 293T (epithelial)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  chicken, human

Y620-p - EphB2 (chicken)
Orthologous residues
EphB2 (human): Y602‑p, EphB2 iso2 (human): Y602‑p, EphB2 (mouse): Y610‑p, EphB2 (rat): Y715‑p, EphB2 (chicken): Y620‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody
 Relevant cell lines - cell types - tissues:  'retina, embryo', 293T (epithelial)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  chicken, human

Y677-p - EphB2 (chicken)
Orthologous residues
EphB2 (human): Y659‑p, EphB2 iso2 (human): Y659‑p, EphB2 (mouse): Y667‑p, EphB2 (rat): Y772‑p, EphB2 (chicken): Y677‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody
 Relevant cell lines - cell types - tissues:  'retina, embryo', 293T (epithelial)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  chicken, human

Y754-p - EphB2 (chicken)
Orthologous residues
EphB2 (human): Y736‑p, EphB2 iso2 (human): Y736‑p, EphB2 (mouse): Y744‑p, EphB2 (rat): Y849‑p, EphB2 (chicken): Y754‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

Y760-p - EphB2 (chicken)
Orthologous residues
EphB2 (human): Y742‑p, EphB2 iso2 (human): Y742‑p, EphB2 (mouse): Y750‑p, EphB2 (rat): Y855‑p, EphB2 (chicken): Y760‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

Y798-p - EphB2 (chicken)
Orthologous residues
EphB2 (human): Y780‑p, EphB2 iso2 (human): Y780‑p, EphB2 (mouse): Y788‑p, EphB2 (rat): Y893‑p, EphB2 (chicken): Y798‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody
 Relevant cell lines - cell types - tissues:  'retina, embryo', 293T (epithelial)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  chicken, human

Y830-p - EphB2 (chicken)
Orthologous residues
EphB2 (human): Y812‑p, EphB2 iso2 (human): Y812‑p, EphB2 (mouse): Y820‑p, EphB2 (rat): Y925‑p, EphB2 (chicken): Y830‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody
 Relevant cell lines - cell types - tissues:  'retina, embryo', 293T (epithelial)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  chicken, human

Y840-p - EphB2 (chicken)
Orthologous residues
EphB2 (human): Y822‑p, EphB2 iso2 (human): Y822‑p, EphB2 (mouse): Y830‑p, EphB2 (rat): Y935‑p, EphB2 (chicken): Y840‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody
 Relevant cell lines - cell types - tissues:  'retina, embryo', 293T (epithelial)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  chicken, human

Y930-p - EphB2 (chicken)
Orthologous residues
EphB2 (human): Y912‑p, EphB2 iso2 (human): Y912‑p, EphB2 (mouse): Y920‑p, EphB2 (rat): Y1025‑p, EphB2 (chicken): Y930‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

Y948-p - EphB2 (chicken)
Orthologous residues
EphB2 (human): Y930‑p, EphB2 iso2 (human): Y930‑p, EphB2 (mouse): Y938‑p, EphB2 (rat): Y1043‑p, EphB2 (chicken): Y948‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, phospho-antibody
 Relevant cell lines - cell types - tissues:  'retina, embryo', 293T (epithelial)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  chicken, human


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