|
Orthologous residues
|
|
AS160 (human): T642‑p, AS160 iso3 (human): T154‑p, AS160 (mouse): T649‑p, AS160 iso2 (mouse): T649‑p, AS160 (rat): T651‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
mutation of modification site
|
|
Cellular systems studied:
cell lines
|
|
Species studied:
hamster
|
|
Upstream Regulation
|
|
Potential in vivo enzymes for site:
|
|
Type
|
Enzyme
|
Evidence
|
Notes
|
|
KINASE
|
Akt1 (human)
|
transfection of constitutively active enzyme
|
|
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
insulin
|
|
InsR (human)
|
|
increase
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
molecular association, regulation
|
|
Modification regulates interactions with:
|
|
Interacting molecule
|
Interacting domains
|
Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
|
|
14-3-3 epsilon (human)
|
|
Induces
|
|
|
co-immunoprecipitation
|
|
14-3-3 theta (human)
|
|
Induces
|
|
|
co-immunoprecipitation
|
|
|
Comments:
phosphorylation of this site has an effect on insulin-stimulated GLUT4 trafficking to the cell surface
|
