Curated Information
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Curated Information Page
PubMed Id: 16669787 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Vitari AC, et al. (2006) Functional interactions of the SPAK/OSR1 kinases with their upstream activator WNK1 and downstream substrate NKCC1. Biochem J 397, 223-31 16669787
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T203-p - NKCC1 (human) ▲
Orthologous residues
NKCC1 (human): T203‑p, NKCC1 (mouse): T196‑p, NKCC1 (rat): T194‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE OSR1 (human)
KINASE STLK3 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE OSR1 (human) transfection of constitutively active enzyme, co-immunoprecipitation, competition with site-specific peptide
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase
Downstream Regulation
 Effect of modification (function):  activation

T207-p - NKCC1 (human) ▲
Orthologous residues
NKCC1 (human): T207‑p, NKCC1 (mouse): T200‑p, NKCC1 (rat): T198‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE OSR1 (human)
KINASE STLK3 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE OSR1 (human) transfection of constitutively active enzyme, co-immunoprecipitation, competition with site-specific peptide
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase
Downstream Regulation
 Effect of modification (function):  activation

T212-p - NKCC1 (human) ▲
Orthologous residues
NKCC1 (human): T212‑p, NKCC1 (mouse): T205‑p, NKCC1 (rat): T203‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE OSR1 (human)
KINASE STLK3 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE OSR1 (human) transfection of constitutively active enzyme, co-immunoprecipitation, competition with site-specific peptide
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase
Downstream Regulation
 Effect of modification (function):  activation


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