Curated Information
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PubMed Id: 16684964 
Heiss E, et al. (2006) Identification of Y589 and Y599 in the juxtamembrane domain of Flt3 as ligand-induced autophosphorylation sites involved in binding of Src family kinases and the protein tyrosine phosphatase SHP2. Blood 108, 1542-50 16684964
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y572-p - FLT3 (human)
Orthologous residues
FLT3 (human): Y572‑p, FLT3 (mouse): Y573‑p, FLT3 iso3 (mouse): Y573‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  32D (myeloid) [IRS1 (mouse)], PAE (endothelial)
 Cellular systems studied:  cell lines
 Species studied:  mouse, pig

Y589-p - FLT3 (human)
Orthologous residues
FLT3 (human): Y589‑p, FLT3 (mouse): Y590‑p, FLT3 iso3 (mouse): Y590‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  32D (myeloid) [IRS1 (mouse)], PAE (endothelial)
 Cellular systems studied:  cell lines
 Species studied:  mouse, pig
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE FLT3 (human)
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
FL increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Src (human) Induces co-immunoprecipitation

Y591-p - FLT3 (human)
Orthologous residues
FLT3 (human): Y591‑p, FLT3 (mouse): Y592‑p, FLT3 iso3 (mouse): Y592‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  32D (myeloid) [IRS1 (mouse)], PAE (endothelial)
 Cellular systems studied:  cell lines
 Species studied:  mouse, pig

Y599-p - FLT3 (human)
Orthologous residues
FLT3 (human): Y599‑p, FLT3 (mouse): Y600‑p, FLT3 iso3 (mouse): Y600‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  32D (myeloid) [IRS1 (mouse)], PAE (endothelial)
 Cellular systems studied:  cell lines
 Species studied:  mouse, pig
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE FLT3 (human)
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
FL increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Src (human) Induces co-immunoprecipitation
SHP-2 (human) Induces co-immunoprecipitation


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