Curated Information
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Curated Information Page
PubMed Id: 12810726 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Khoo S, et al. (2003) Regulation of insulin gene transcription by ERK1 and ERK2 in pancreatic beta cells. J Biol Chem 278, 32969-77 12810726
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S352-p - E2A iso2 (human) ▲
Orthologous residues
E2A (human): S352‑p, E2A iso2 (human): S352‑p, E2A (mouse): S351‑p, E2A iso2 (mouse): S350‑p
Characterization
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ERK2 (human)
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
NEUROD1 (mouse) Induces transcription, altered in vitro

T355-p - E2A iso2 (human) ▲
Orthologous residues
E2A (human): T355‑p, E2A iso2 (human): T355‑p, E2A (mouse): T354‑p, E2A iso2 (mouse): T353‑p
Characterization
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ERK2 (human)
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
NEUROD1 (mouse) Induces transcription, altered in vitro

S359-p - E2A iso2 (human) ▲
Orthologous residues
E2A (human): S359‑p, E2A iso2 (human): S359‑p, E2A (mouse): S358‑p, E2A iso2 (mouse): S357‑p
Characterization
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ERK2 (human)
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
NEUROD1 (mouse) Induces transcription, altered in vitro

S61-p - PDX1 (human) ▲
Orthologous residues
PDX1 (human): S61‑p, PDX1 (mouse): S61‑p, PDX1 (rat): S61‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site
 Relevant cell lines - cell types - tissues:  beta TC3 (pancreatic)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ERK2 (human)
KINASE JNK2 (human)
KINASE p38-alpha (human)
Downstream Regulation
 Effect of modification (process):  transcription, altered

S66-p - PDX1 (human) ▲
Orthologous residues
PDX1 (human): S66‑p, PDX1 (mouse): S66‑p, PDX1 (rat): S66‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site
 Relevant cell lines - cell types - tissues:  beta TC3 (pancreatic)
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE p38-alpha (human)
KINASE ERK2 (human)
KINASE JNK2 (human)
Downstream Regulation
 Effect of modification (process):  transcription, altered

S162-p - NEUROD1 (mouse) ▲
Orthologous residues
NEUROD1 (human): S162‑p, NEUROD1 (mouse): S162‑p, NEUROD1 (rat): S162‑p
Characterization
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ERK2 (human)

S259-p - NEUROD1 (mouse) ▲
Orthologous residues
NEUROD1 (human): S259‑p, NEUROD1 (mouse): S259‑p, NEUROD1 (rat): S259‑p
Characterization
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ERK2 (human)

S266-p - NEUROD1 (mouse) ▲
Orthologous residues
NEUROD1 (human): S266‑p, NEUROD1 (mouse): S266‑p, NEUROD1 (rat): S266‑p
Characterization
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ERK2 (human)

S274-p - NEUROD1 (mouse) ▲
Orthologous residues
NEUROD1 (human): S274‑p, NEUROD1 (mouse): S274‑p, NEUROD1 (rat): S274‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift
 Relevant cell lines - cell types - tissues:  INS-1 (pancreatic)
 Cellular systems studied:  cell lines
 Species studied:  rat
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ERK2 (human)
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
E2A iso2 (human) Induces transcription, altered in vitro


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