Curated Information
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Curated Information Page
PubMed Id: 8668214 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Tanaka T, et al. (1996) The extracellular signal-regulated kinase pathway phosphorylates AML1, an acute myeloid leukemia gene product, and potentially regulates its transactivation ability. Mol Cell Biol 16, 3967-79 8668214
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S249-p - AML1 (human)
Orthologous residues
AML1 (human): S249‑p, AML1 iso2 (human): S249‑p, AML1 iso8 (human): S276‑p, AML1 (mouse): S249‑p, AML1 (rat): S249‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phosphoamino acid analysis, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK1 (rat)
KINASE ERK2 (rat)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK1 (rat) co-immunoprecipitation, mutation in upstream enzyme recognition motif, activation of upstream enzyme
KINASE ERK2 (rat) co-immunoprecipitation, mutation in upstream enzyme recognition motif, activation of upstream enzyme
Downstream Regulation
 Effect of modification (function):  activity, induced
 Effect of modification (process):  cell growth, altered, transcription, altered

S266-p - AML1 (human)
Orthologous residues
AML1 (human): S266‑p, AML1 iso2 (human): S266‑p, AML1 iso8 (human): S293‑p, AML1 (mouse): S266‑p, AML1 (rat): S266‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phosphoamino acid analysis, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (rat)
KINASE ERK1 (rat)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK1 (rat) co-immunoprecipitation, mutation in upstream enzyme recognition motif, activation of upstream enzyme
KINASE ERK2 (rat) co-immunoprecipitation, mutation in upstream enzyme recognition motif, activation of upstream enzyme
Downstream Regulation
 Effect of modification (function):  activity, induced
 Effect of modification (process):  cell growth, altered, transcription, altered

T273-p - AML1 (human)
Orthologous residues
AML1 (human): T273‑p, AML1 iso2 (human): T273‑p, AML1 iso8 (human): T300‑p, AML1 (mouse): T273‑p, AML1 (rat): T272‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phosphoamino acid analysis, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

S276-p - AML1 (human)
Orthologous residues
AML1 (human): S276‑p, AML1 iso2 (human): S276‑p, AML1 iso8 (human): S303‑p, AML1 (mouse): S276‑p, AML1 (rat): S275‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phosphoamino acid analysis, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey


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