|
Orthologous residues
|
|
ZAP70 (human): Y319‑p, ZAP70 (mouse): Y318‑p, ZAP70 (rat): Y314‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
mutation of modification site, phospho-antibody, western blotting
|
|
Relevant cell lines - cell types - tissues:
COS (fibroblast), Jurkat (T lymphocyte), P116 (T lymphocyte)
|
|
Cellular systems studied:
cell lines
|
|
Species studied:
human, monkey
|
|
Upstream Regulation
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
anti-CD3
|
|
|
|
increase
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
activation, molecular association, regulation, phosphorylation
|
|
Effect of modification (process):
transcription, altered
|
|
Modification regulates interactions with:
|
|
Interacting molecule
|
Interacting domains
|
Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
|
|
PLCG1 (human)
|
SH2
|
Induces
|
activation
|
|
pull-down assay
|
|
Lck (human)
|
SH2
|
Induces
|
activation
|
|
pull-down assay
|
|
|
Comments:
Ca(2+) mobilization
|
