Curated Information
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Curated Information Page
PubMed Id: 16420481 
Manceau V, et al. (2006) Major phosphorylation of SF1 on adjacent Ser-Pro motifs enhances interaction with U2AF65. FEBS J 273, 577-87 16420481
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S80-p - SF1 (human)
Orthologous residues
SF1 (human): S80‑p, SF1 iso2 (human): S80‑p, SF1 iso4 (human): S80‑p, SF1 iso5 (human): S205‑p, SF1 iso6 (human): S80‑p, SF1 (mouse): S80‑p, SF1 iso2 (mouse): S80‑p, SF1 (rat): S80‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial), WEHI-231 (B lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE KIS (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE KIS (human) transfection of inactive enzyme
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  RNA splicing, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
U2AF2 (human) Induces RNA splicing, altered co-immunoprecipitation
 Comments:  facilitates formation of the SF1-U2AF-RNA complex

S82-p - SF1 (human)
Orthologous residues
SF1 (human): S82‑p, SF1 iso2 (human): S82‑p, SF1 iso4 (human): S82‑p, SF1 iso5 (human): S207‑p, SF1 iso6 (human): S82‑p, SF1 (mouse): S82‑p, SF1 iso2 (mouse): S82‑p, SF1 (rat): S82‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial), WEHI-231 (B lymphocyte)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE KIS (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE KIS (human) transfection of inactive enzyme
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  RNA splicing, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
U2AF2 (human) Induces RNA splicing, altered co-immunoprecipitation
 Comments:  facilitates formation of the SF1-U2AF-RNA complex


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