Curated Information
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Curated Information Page
PubMed Id: 9685341 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Smolenski A, et al. (1998) Analysis and regulation of vasodilator-stimulated phosphoprotein serine 239 phosphorylation in vitro and in intact cells using a phosphospecific monoclonal antibody. J Biol Chem 273, 20029-35 9685341
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S157-p - VASP (human)
Orthologous residues
VASP (human): S157‑p, VASP (mouse): S153‑p, VASP (rat): S154‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, mutation of modification site
 Relevant cell lines - cell types - tissues:  platelet-blood, PtK2 (epithelial)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human, rat
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKACA (cow)
KINASE PKG1 (cow)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PGI2 increase
SNP increase
5 6-DCl-cBIMPS increase
cGMP analog increase
PGE1 increase

S239-p - VASP (human)
Orthologous residues
VASP (human): S239‑p, VASP (mouse): S235‑p, VASP (rat): S236‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  platelet-blood, PtK2 (epithelial)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human, rat
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKG1 (cow)
KINASE PKACA (cow)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PGI2 increase
SNP increase
5 6-DCl-cBIMPS increase
cGMP analog increase
PGE1 increase


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