|
Orthologous residues
|
|
L‑plastin (human): S5‑p, L‑plastin (mouse): S5‑p, L‑plastin (rat): S5‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
mutation of modification site, phospho-antibody
|
|
Relevant cell lines - cell types - tissues:
293T (epithelial), Jurkat (T lymphocyte), Vero (epithelial)
|
|
Cellular systems studied:
cell lines
|
|
Species studied:
monkey
|
|
Enzymes shown to modify site in vitro:
|
|
|
|
Upstream Regulation
|
|
Potential in vivo enzymes for site:
|
|
Type
|
Enzyme
|
Evidence
|
Notes
|
|
KINASE
|
PKACA (human)
|
pharmacological activator of upstream enzyme
|
|
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
8-Rp-cAMP
|
|
|
|
increase
|
|
|
H-89
|
8-Rp-cAMP
|
|
|
inhibit treatment-induced increase
|
|
|
forskolin
|
|
|
|
increase
|
|
|
H-89
|
forskolin
|
|
|
inhibit treatment-induced increase
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
molecular association, regulation
|
|
Modification regulates interactions with:
|
|
Interacting molecule
|
Interacting domains
|
Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
|
|
ACTA1 (human)
|
|
Induces
|
|
|
microscopy-colocalization, in vitro
|
|
|
Comments:
interaction with F-actin enhance cells migration and invasion
|
