Curated Information
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Curated Information Page
PubMed Id: 10504341 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Wells NJ, et al. (1999) The C-terminal domain of the Cdc2 inhibitory kinase Myt1 interacts with Cdc2 complexes and is required for inhibition of G(2)/M progression. J Cell Sci 112 ( Pt 19), 3361-71 10504341
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T14-p - CDK1 (human) ▲
Orthologous residues
CDK1 (human): T14‑p, CDK1 (mouse): T14‑p, CDK1 (rat): T14‑p, CDK1 (chicken): T14‑p, CDK1 (fruit fly): T14‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Myt1 (human) co-immunoprecipitation, transfection of inactive enzyme, transfection of wild-type enzyme
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited
 Effect of modification (process):  cell cycle regulation

Y15-p - CDK1 (human) ▲
Orthologous residues
CDK1 (human): Y15‑p, CDK1 (mouse): Y15‑p, CDK1 (rat): Y15‑p, CDK1 (chicken): Y15‑p, CDK1 (fruit fly): Y15‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Myt1 (human) co-immunoprecipitation, transfection of inactive enzyme, transfection of wild-type enzyme
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited
 Effect of modification (process):  cell cycle regulation

T412-p - Myt1 (human) ▲
Orthologous residues
Myt1 (human): T412‑p, Myt1 (mouse): T403‑p, Myt1 (starfish): T419‑p
Characterization
 Methods used to characterize site in vivo:  flow cytometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Pin1 (human) Induces co-immunoprecipitation, pull-down assay

S416-p - Myt1 (human) ▲
Orthologous residues
Myt1 (human): S416‑p, Myt1 (mouse): S407‑p, Myt1 (starfish): ‑
Characterization
 Methods used to characterize site in vivo:  flow cytometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Pin1 (human) Induces co-immunoprecipitation, pull-down assay

S441-p - Myt1 (human) ▲
Orthologous residues
Myt1 (human): S441‑p, Myt1 (mouse): S432‑p, Myt1 (starfish): S462‑p
Characterization
 Methods used to characterize site in vivo:  flow cytometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Pin1 (human) Induces co-immunoprecipitation, pull-down assay

T455-p - Myt1 (human) ▲
Orthologous residues
Myt1 (human): T455‑p, Myt1 (mouse): T446‑p, Myt1 (starfish): ‑
Characterization
 Methods used to characterize site in vivo:  flow cytometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Pin1 (human) Induces co-immunoprecipitation, pull-down assay

T461-p - Myt1 (human) ▲
Orthologous residues
Myt1 (human): T461‑p, Myt1 (mouse): I452‑p, Myt1 (starfish): T500‑p
Characterization
 Methods used to characterize site in vivo:  flow cytometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Pin1 (human) Induces co-immunoprecipitation, pull-down assay


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