Curated Information

Javascript is not enabled on this browser. This site will not work properly without Javascript.

Home

Curated Information Page

PubMed Id: 19477150

This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus^®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus^®, click here.

Chen BS, Roche KW (2009) Growth factor-dependent trafficking of cerebellar NMDA receptors via protein kinase B/Akt phosphorylation of NR2C. Neuron 62, 471-8 19477150

Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.

S1084-p - NMDAR2C (mouse)

▲

Orthologous residues

NMDAR2C (human): S1081‑p, NMDAR2C (mouse): S1084‑p, NMDAR2C (rat): S1083‑p

Characterization

Methods used to characterize site in vivo: phospho-antibody, western blotting

Relevant cell lines - cell types - tissues: 'brain, cerebellum'

Cellular systems studied: tissue

Species studied: mouse

Upstream Regulation

Potential in vivo enzymes for site:

Type	Enzyme	Evidence	Notes
KINASE	Akt1 (human)	pharmacological activator of upstream enzyme

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
IGF-1				increase
NMDA				increase
APV				decrease

Downstream Regulation

Effect of modification (function): intracellular localization

Effect of modification (process): apoptosis, altered

Comments: important for cell survival

S1083-p - NMDAR2C (rat)

▲

Orthologous residues

NMDAR2C (human): S1081‑p, NMDAR2C (mouse): S1084‑p, NMDAR2C (rat): S1083‑p

Characterization

Methods used to characterize site in vivo: immunoprecipitation, mutation of modification site, phospho-antibody, western blotting

Relevant cell lines - cell types - tissues: 293 (epithelial)

Cellular systems studied: cell lines

Species studied: human

Enzymes shown to modify site in vitro:

Type	Enzyme
KINASE	Akt1 (human)

Upstream Regulation

Potential in vivo enzymes for site:

Type	Enzyme	Evidence	Notes
KINASE	Akt1 (human)	pharmacological activator of upstream enzyme
KINASE	Akt1 (human)	transfection of dominant-negative enzyme, transfection of constitutively active enzyme

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
IGF-1				increase
wortmannin	IGF-1			inhibit treatment-induced increase

Downstream Regulation

Effect of modification (function): intracellular localization, molecular association, regulation

Modification regulates interactions with:

Interacting molecule	Interacting domains	Effect	Consequences (function)	Consequences (process)	Detection assays
14-3-3 epsilon (rat)		Induces			yeast two-hybrid, co-immunoprecipitation

Home \| Curator Login	With enhanced literature mining using Linguamatics I2E		Produced by 3rd Millennium \| Design by Digizyme
			©2003-2013 Cell Signaling Technology, Inc.