Curated Information
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Curated Information Page
PubMed Id: 19560418 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Dougherty MK, et al. (2009) KSR2 is a calcineurin substrate that promotes ERK cascade activation in response to calcium signals. Mol Cell 34, 652-62 19560418
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S198-p - KSR2 iso2 (mouse)
Orthologous residues
KSR2 (human): S198‑p, KSR2 (mouse): S198‑p, KSR2 iso2 (mouse): S198‑p, KSR2 (rat): S198‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site, phosphoamino acid analysis, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), NG108
 Cellular systems studied:  cell lines
 Species studied:  human, monkey
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP3CA (human) pharmacological inhibitor of upstream enzyme, co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionomycin, phorbol ester decrease
CsA ionomycin, phorbol ester inhibit treatment-induced decrease
KCl decrease
CsA KCl inhibit treatment-induced decrease
glucose decrease
CsA glucose inhibit treatment-induced decrease

T287-p - KSR2 iso2 (mouse)
Orthologous residues
KSR2 (human): T290‑p, KSR2 (mouse): T291‑p, KSR2 iso2 (mouse): T287‑p, KSR2 (rat): T290‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site, phosphoamino acid analysis, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), NG108
 Cellular systems studied:  cell lines
 Species studied:  human, monkey
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP3CA (human) pharmacological inhibitor of upstream enzyme, co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionomycin, phorbol ester decrease
CsA ionomycin, phorbol ester inhibit treatment-induced decrease
KCl decrease
CsA KCl inhibit treatment-induced decrease
glucose decrease
CsA glucose inhibit treatment-induced decrease

S310-p - KSR2 iso2 (mouse)
Orthologous residues
KSR2 (human): S313‑p, KSR2 (mouse): S314‑p, KSR2 iso2 (mouse): S310‑p, KSR2 (rat): S313‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site, phosphoamino acid analysis, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), NG108
 Cellular systems studied:  cell lines
 Species studied:  human, monkey
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP3CA (human) pharmacological inhibitor of upstream enzyme, co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionomycin, phorbol ester decrease
CsA ionomycin, phorbol ester inhibit treatment-induced decrease
KCl decrease
CsA KCl inhibit treatment-induced decrease
glucose decrease
CsA glucose inhibit treatment-induced decrease
Downstream Regulation
 Effect of modification (function):  intracellular localization, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces

S471-p - KSR2 iso2 (mouse)
Orthologous residues
KSR2 (human): S474‑p, KSR2 (mouse): S484‑p, KSR2 iso2 (mouse): S471‑p, KSR2 (rat): S483‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site, phosphoamino acid analysis, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), NG108
 Cellular systems studied:  cell lines
 Species studied:  human, monkey
Downstream Regulation
 Effect of modification (function):  intracellular localization, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces

S487-p - KSR2 iso2 (mouse)
Orthologous residues
KSR2 (human): S490‑p, KSR2 (mouse): S500‑p, KSR2 iso2 (mouse): S487‑p, KSR2 (rat): S499‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site, phosphoamino acid analysis, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), NG108
 Cellular systems studied:  cell lines
 Species studied:  human, monkey

T494-p - KSR2 iso2 (mouse)
Orthologous residues
KSR2 (human): T497‑p, KSR2 (mouse): T507‑p, KSR2 iso2 (mouse): T494‑p, KSR2 (rat): T506‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site, phosphoamino acid analysis, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), NG108
 Cellular systems studied:  cell lines
 Species studied:  human, monkey

S625-p - KSR2 iso2 (mouse)
Orthologous residues
KSR2 (human): S628‑p, KSR2 (mouse): S638‑p, KSR2 iso2 (mouse): S625‑p, KSR2 (rat): S637‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site, phosphoamino acid analysis, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), NG108
 Cellular systems studied:  cell lines
 Species studied:  human, monkey


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