Curated Information
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PubMed Id: 9136927 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Kretzschmar M, et al. (1997) The TGF-beta family mediator Smad1 is phosphorylated directly and activated functionally by the BMP receptor kinase. Genes Dev 11, 984-95 9136927
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S462-p - Smad1 (human)
Orthologous residues
Smad1 (human): S462‑p, Smad1 (mouse): S462‑p, Smad1 (rat): S465‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphoamino acid analysis, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  COS (fibroblast), R-1B/L17 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  mink, monkey
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE BMPR1B (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE BMPR1B (human) transfection of wild-type enzyme, co-immunoprecipitation
Downstream Regulation
 Effect of modification (function):  activity, induced, intracellular localization, molecular association, regulation
 Effect of modification (process):  transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Smad4 (human) Induces co-immunoprecipitation

S463-p - Smad1 (human)
Orthologous residues
Smad1 (human): S463‑p, Smad1 (mouse): S463‑p, Smad1 (rat): S466‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphoamino acid analysis, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  COS (fibroblast), R-1B/L17 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  mink, monkey
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE BMPR1B (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE BMPR1B (human) transfection of wild-type enzyme, co-immunoprecipitation
Downstream Regulation
 Effect of modification (function):  activity, induced, intracellular localization, molecular association, regulation
 Effect of modification (process):  transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Smad4 (human) Induces co-immunoprecipitation

S465-p - Smad1 (human)
Orthologous residues
Smad1 (human): S465‑p, Smad1 (mouse): S465‑p, Smad1 (rat): S468‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphoamino acid analysis, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  COS (fibroblast), R-1B/L17 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  mink, monkey
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE BMPR1B (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE BMPR1B (human) transfection of wild-type enzyme, co-immunoprecipitation
Downstream Regulation
 Effect of modification (function):  activity, induced, intracellular localization, molecular association, regulation
 Effect of modification (process):  transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Smad4 (human) Induces co-immunoprecipitation

S464-p - Smad2 (human)
Orthologous residues
Smad2 (human): S464‑p, Smad2 (mouse): S464‑p, Smad2 (rat): S464‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphoamino acid analysis, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  COS (fibroblast), R-1B/L17 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  mink, monkey
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE BMPR1B (human) transfection of wild-type enzyme, co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TGF-beta increase

S465-p - Smad2 (human)
Orthologous residues
Smad2 (human): S465‑p, Smad2 (mouse): S465‑p, Smad2 (rat): S465‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphoamino acid analysis, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  COS (fibroblast), R-1B/L17 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  mink, monkey
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE BMPR1B (human) transfection of wild-type enzyme, co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TGF-beta increase

S467-p - Smad2 (human)
Orthologous residues
Smad2 (human): S467‑p, Smad2 (mouse): S467‑p, Smad2 (rat): S467‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphoamino acid analysis, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  COS (fibroblast), R-1B/L17 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  mink, monkey
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE BMPR1B (human) transfection of wild-type enzyme, co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TGF-beta increase


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