Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Curated Information Page
PubMed Id: 16497729 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Kim MY, et al. (2006) Acetylation of estrogen receptor alpha by p300 at lysines 266 and 268 enhances the deoxyribonucleic acid binding and transactivation activities of the receptor. Mol Endocrinol 20, 1479-93 16497729
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

K244-ac - ER-alpha (human)
Orthologous residues
ER‑alpha (human): K244‑ac, ER‑alpha (mouse): K248‑ac, ER‑alpha (rat): K249‑ac
Characterization
 Methods used to characterize site in vivo mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE p300 (human)

K252-ac - ER-alpha (human)
Orthologous residues
ER‑alpha (human): K252‑ac, ER‑alpha (mouse): K256‑ac, ER‑alpha (rat): K257‑ac
Characterization
 Methods used to characterize site in vivo mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE p300 (human)

K266-ac - ER-alpha (human)
Orthologous residues
ER‑alpha (human): K266‑ac, ER‑alpha (mouse): K270‑ac, ER‑alpha (rat): K271‑ac
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  293T (epithelial), MDA-MB231 (breast cell) [ER-alpha (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE p300 (human)
DEACETYLASE SIRT1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
estrogen increase
Downstream Regulation
 Effect of modification (function):  activity, induced, molecular association, regulation
 Effect of modification (process):  transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DNA Induces

K268-ac - ER-alpha (human)
Orthologous residues
ER‑alpha (human): K268‑ac, ER‑alpha (mouse): K272‑ac, ER‑alpha (rat): K273‑ac
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  breast cancer
 Relevant cell lines - cell types - tissues:  293T (epithelial), MDA-MB231 (breast cell) [ER-alpha (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
DEACETYLASE SIRT1 (human)
ACETYLTRANSFERASE p300 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
estrogen increase
Downstream Regulation
 Effect of modification (function):  activity, induced, molecular association, regulation
 Effect of modification (process):  transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DNA Induces

K299-ac - ER-alpha (human)
Orthologous residues
ER‑alpha (human): K299‑ac, ER‑alpha (mouse): K303‑ac, ER‑alpha (rat): K304‑ac
Characterization
 Methods used to characterize site in vivo mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE p300 (human)


Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.