Curated Information
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Curated Information Page
PubMed Id: 11062067 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Fleming Y, et al. (2000) Synergistic activation of stress-activated protein kinase 1/c-Jun N-terminal kinase (SAPK1/JNK) isoforms by mitogen-activated protein kinase kinase 4 (MKK4) and MKK7. Biochem J 352 Pt 1, 145-54 11062067
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T183-p - JNK1 iso2 (human) ▲
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  cervical cancer, cervical squamous cell carcinoma
 Relevant cell lines - cell types - tissues:  293 (epithelial), KB (squamous)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE MKK7 (human)
KINASE MKK7 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anisomycin increase
IL-1a increase
UV increase
osmotic stress increase
TNF increase

Y185-p - JNK1 iso2 (human) ▲
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  cervical cancer, cervical squamous cell carcinoma
 Relevant cell lines - cell types - tissues:  293 (epithelial), KB (squamous)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE MKK4 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anisomycin increase
IL-1a increase
UV increase
osmotic stress increase
TNF increase

T183-p - JNK2 (human) ▲
Orthologous residues
JNK2 (human): T183‑p, JNK2 iso2 (human): T183‑p, JNK2 iso3 (human): T183‑p, JNK2 (mouse): T183‑p, JNK2 (rat): T183‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  cervical cancer, cervical squamous cell carcinoma
 Relevant cell lines - cell types - tissues:  293 (epithelial), KB (squamous)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE MKK7 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anisomycin increase
IL-1a increase
UV increase
osmotic stress increase
TNF increase

Y185-p - JNK2 (human) ▲
Orthologous residues
JNK2 (human): Y185‑p, JNK2 iso2 (human): Y185‑p, JNK2 iso3 (human): Y185‑p, JNK2 (mouse): Y185‑p, JNK2 (rat): Y185‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  cervical cancer, cervical squamous cell carcinoma
 Relevant cell lines - cell types - tissues:  293 (epithelial), KB (squamous)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE MKK4 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anisomycin increase
IL-1a increase
UV increase
osmotic stress increase
TNF increase

T404-p - JNK2 (human) ▲
Orthologous residues
JNK2 (human): T404‑p, JNK2 iso2 (human): ‑, JNK2 iso3 (human): ‑, JNK2 (mouse): T403‑p, JNK2 (rat): T403‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  cervical cancer, cervical squamous cell carcinoma
 Relevant cell lines - cell types - tissues:  293 (epithelial), KB (squamous)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE MKK7 (human)
KINASE CK2-A1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anisomycin no change compared to control
IL-1a no change compared to control
UV no change compared to control
osmotic stress increase
TNF no change compared to control
MKK7 (human) increase

S407-p - JNK2 (human) ▲
Orthologous residues
JNK2 (human): S407‑p, JNK2 iso2 (human): ‑, JNK2 iso3 (human): ‑, JNK2 (mouse): S406‑p, JNK2 (rat): S406‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  cervical cancer, cervical squamous cell carcinoma
 Relevant cell lines - cell types - tissues:  293 (epithelial), KB (squamous)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE MKK7 (human)
KINASE CK2-A1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anisomycin no change compared to control
IL-1a no change compared to control
UV no change compared to control
osmotic stress increase
TNF no change compared to control
MKK7 (human) increase

T221-p - JNK3 iso2 (human) ▲
Orthologous residues
JNK3 (human): T221‑p, JNK3 iso2 (human): T221‑p, JNK3 (mouse): T221‑p, JNK3 iso2 (mouse): T221‑p, JNK3 iso3 (mouse): ‑, JNK3 (rat): T221‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  cervical cancer, cervical squamous cell carcinoma
 Relevant cell lines - cell types - tissues:  293 (epithelial), KB (squamous)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE MKK7 (human)

Y223-p - JNK3 iso2 (human) ▲
Orthologous residues
JNK3 (human): Y223‑p, JNK3 iso2 (human): Y223‑p, JNK3 (mouse): Y223‑p, JNK3 iso2 (mouse): Y223‑p, JNK3 iso3 (mouse): ‑, JNK3 (rat): Y223‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Disease tissue studied:  cervical cancer, cervical squamous cell carcinoma
 Relevant cell lines - cell types - tissues:  293 (epithelial), KB (squamous)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE MKK4 (human)


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