Curated Information
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Curated Information Page
PubMed Id: 12665569 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Jones N, et al. (2003) A unique autophosphorylation site on Tie2/Tek mediates Dok-R phosphotyrosine binding domain binding and function. Mol Cell Biol 23, 2658-68 12665569
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y1100-p - TIE2 (mouse)
Orthologous residues
TIE2 (human): Y1102‑p, TIE2 (mouse): Y1100‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey, mouse
 Comments:  EA.hy926 endothelial cells, Tie2-delta endothelioma cell line

Y1106-p - TIE2 (mouse)
Orthologous residues
TIE2 (human): Y1108‑p, TIE2 (mouse): Y1106‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey, mouse
 Comments:  EA.hy926 endothelial cells, Tie2-delta endothelioma cell line
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
angiopoietin-1 increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, phosphorylation
 Effect of modification (process):  cell motility, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Dok2 (human) PTB Induces co-immunoprecipitation
 Comments:  Mutation of Y1106F reduces tyrosine phosphorylation on Dok-R (Dok2). Mutation prevents Ang1-MH-induced chemotaxis (cannot rescue the null).


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