Curated Information
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Curated Information Page
PubMed Id: 14532121 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Pitson SM, et al. (2003) Activation of sphingosine kinase 1 by ERK1/2-mediated phosphorylation. EMBO J 22, 5491-500 14532121
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S225-p - SPHK1 (human)
Orthologous residues
SPHK1 (human): S225‑p, SPHK1 iso2 (human): S311‑p, SPHK1 (mouse): S225‑p, SPHK1 (rat): S225‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK1 (human) pharmacological inhibitor of upstream enzyme, co-immunoprecipitation
KINASE ERK2 (human) pharmacological inhibitor of upstream enzyme, co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
PD98059 TNF inhibit treatment-induced increase
U0126 TNF inhibit treatment-induced increase
phorbol ester increase
PD98059 phorbol ester inhibit treatment-induced increase
U0126 phorbol ester inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, intracellular localization


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